Whole Ribosome NMR: Dipolar Couplings and Contributions to Whole Cells.
 

Whole Ribosome NMR: Dipolar Couplings and Contributions to Whole Cells.

Related Articles Whole Ribosome NMR: Dipolar Couplings and Contributions to Whole Cells.

J Phys Chem B. 2017 Sep 13;:

Authors: Nygaard R, Romaniuk JAH, Rice DM, Cegelski L

Abstract
Solid-state NMR is a powerful tool for quantifying chemical composition and structure in complex assemblies and even whole cells. We employed N{P} REDOR NMR to obtain atomic-level distance propensities in intact 15N-labeled E. coli ribosomes. The experimental REDOR dephasing of shift-resolved lysyl amine nitrogens by phosphorous was comparable to that expected from a calculation of N-P distances involving the lysines included in the crystal structure coordinates. Among the nitrogen contributions to the REDOR spectra, the strongest dephasing emerged from the dipolar couplings to phosphorous involving nitrogen peaks ascribed primarily to rRNA and the weakest dephasing arose from protein amide nitrogens. This approach is applicable to any macromolecular system and provides quantitative comparisons of distance proximities between shift-resolved nuclei of one type and heteronuclear dephasing spins. Enhanced molecular specificity could be achieved through the use of spectroscopic filters or specific labeling. Furthermore, ribosome 13C and 15N CPMAS spectra were compared with those of whole cells from which the ribosomes were isolated. Whole-cell signatures of ribosomes were identified and should be of value in comparing overall cellular ribosome content in whole-cell samples.


PMID: 28901760 [PubMed - as supplied by publisher]



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