Related ArticlesIn vivo detection of the cyclic osmoregulated periplasmic glucan of Ralstonia solanacearum by high-resolution magic angle spinning NMR.
J Magn Reson. 2001 Jul;151(1):118-23
Authors: Wieruszeski JM, Bohin A, Bohin JP, Lippens G
We investigate the mobility of the osmoregulated periplasmic glucans of Ralstonia solanacearum in the bacterial periplasm through the use of high-resolution (HR) NMR spectroscopy under static and magic angle spinning (MAS) conditions. Because the nature of periplasm is far from an isotropic aqueous solution, the molecules could be freely diffusing or rather associated to a periplasmic protein, a membrane protein, a lipid, or the peptidoglycan. HR MAS NMR spectroscopy leads to more reproducible results and allows the in vivo detection and characterization of the complex molecule.
[Stan NMR blog] Mercury in-vivo imaging?
Mercury in-vivo imaging?
Answer to a query about the feasibility of 199Hg MRI
Source: Stan blog library
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12-21-2010 02:14 AM
[NMR paper] Detection of the protein-protein interaction between cyclic AMP receptor protein and
Detection of the protein-protein interaction between cyclic AMP receptor protein and RNA polymerase, by (13)C-carbonyl NMR.
Related Articles Detection of the protein-protein interaction between cyclic AMP receptor protein and RNA polymerase, by (13)C-carbonyl NMR.
J Biochem. 2001 Jul;130(1):57-61
Authors: Lee TW, Won HS, Park SH, Kyogoku Y, Lee BJ
Cyclic AMP receptor protein (CRP) plays a key role in the transcription regulation of many prokaryotic genes. Upon the binding of cyclic AMP, CRP is allosterically activated, binds to target DNA...
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11-19-2010 08:44 PM
[NMR paper] NMR solution structure of the periplasmic chaperone FimC.
NMR solution structure of the periplasmic chaperone FimC.
Related Articles NMR solution structure of the periplasmic chaperone FimC.
Nat Struct Biol. 1998 Oct;5(10):885-90
Authors: Pellecchia M, Güntert P, Glockshuber R, Wüthrich K
The NMR structure of the 205-residue periplasmic chaperone FimC is presented. This protein consists of two globular domains with immunoglobulin-like folds connected by a 15-residue linker peptide. The relative orientation of the two domains is defined by hydrophobic contacts and an interdomain salt bridge. FimC...
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11-17-2010 11:15 PM
[NMR analysis blog] Conformational analysis of cyclic compounds using Mspin and RDCs
Conformational analysis of cyclic compounds using Mspin and RDCs
On the occasion of the release of a new version of Mspin (BTW, this is the very first multiplatform version of Mspin: it works now in Windows, Mac OS X and Linux), I would like to bring into your attention one of the many applications where this software plays an instrumental role: The application of Mspin to the study of seven-membered rings compounds by NMR.
The NMR study of seven-membered ring compounds is a classical problem in conformational analysis. They are commonly studied by means of NOE-based experiments...
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10-24-2010 08:03 AM
[NMR paper] Cyclic voltammetry and 1H-NMR of Rhodopseudomonas palustris cytochrome c2. Probing su
Cyclic voltammetry and 1H-NMR of Rhodopseudomonas palustris cytochrome c2. Probing surface charges through anion-binding studies.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Cyclic voltammetry and 1H-NMR of Rhodopseudomonas palustris cytochrome c2. Probing surface charges through anion-binding studies.
Eur J Biochem. 1995 Oct 1;233(1):335-9
Authors: Battistuzzi G, Borsari M, Dallari D, Ferretti S, Sola M
The effects of...
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08-22-2010 03:50 AM
[NMR paper] Crystal structure and NMR conformation of a cyclic pseudotetrapeptide containing uret
Crystal structure and NMR conformation of a cyclic pseudotetrapeptide containing urethane backbone linkages.
Related Articles Crystal structure and NMR conformation of a cyclic pseudotetrapeptide containing urethane backbone linkages.
Biopolymers. 1994 Mar;34(3):403-14
Authors: Parkinson GN, Wu Y, Fan P, Kohn J, Baum J, Berman HM
Urethane bonds, derived from the hydroxyl group of the tyrosine side chain, have been investigated as a new type of amide bond mimetic in the design of pseudopeptides. The structure of a representative cyclic...
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08-22-2010 03:33 AM
[NMR paper] In vivo detection and characterization of protein adducts resulting from bioactivatio
In vivo detection and characterization of protein adducts resulting from bioactivation of haloethene cysteine S-conjugates by 19F NMR: chlorotrifluoroethene and tetrafluoroethene.
Related Articles In vivo detection and characterization of protein adducts resulting from bioactivation of haloethene cysteine S-conjugates by 19F NMR: chlorotrifluoroethene and tetrafluoroethene.
Chem Res Toxicol. 1992 Jan-Feb;5(1):34-41
Authors: Harris JW, Dekant W, Anders MW
Several haloalkenes are selective nephrotoxins. The bioactivation of nephrotoxic...
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08-21-2010 11:41 PM
In vivo protein NMR assignments by PR-NMR
Multidimensional NMR Spectroscopy for Protein Characterization and Assignment inside Cells
Patrick N. Reardon and Leonard D. Spicer*
Departments of Biochemistry and Radiology, Duke University Medical Center, Durham, North Carolina 27710
J. Am. Chem. Soc., 127 (31), 10848 -10849, 2005.
http://pubs.acs.org/isubscribe/journals/jacsat/127/i31/figures/ja053145kn00001.gif
Abstract:
High-field, heteronuclear NMR spectroscopy of biological macromolecules in native cellular environments is limited by the low concentrations present and the long data acquisition times needed for the...
In vivo detection of the cyclic osmoregulated periplasmic glucan of Ralstonia solanac
Linear Mode
