In vivo 133Cs-NMR a probe for studying subcellular compartmentation and ion uptake in
 

In vivo 133Cs-NMR a probe for studying subcellular compartmentation and ion uptake in maize root tissue.

http://www.ncbi.nlm.nih.gov/corehtml...PubMedLink.gif Related Articles In vivo 133Cs-NMR a probe for studying subcellular compartmentation and ion uptake in maize root tissue.

Biochim Biophys Acta. 1990 Sep 1;1054(2):169-75

Authors: Pfeffer PE, Rolin DB, Brauer D, Tu SI, Kumosinski TF

Three 133Cs-NMR signals were observed in the spectra of CsCl-perfused and CsCl-grown maize seedling root tips. Two relatively broad lower field resonances were assigned to the subcellular, compartmented Cs+ in the cytoplasm and vacuole, respectively. The rate of area increase of the broader cytoplasmic Cs resonance was about 9-times faster than that of the vacuolar signal during the first 300 min of tissue perfusion with CsCl. In addition, the spin lattice relaxation time of the cytoplasmic Cs resonance was approx. 3-times shorter than that of the extracellular resonance, while the Cs+ signal associated with the metabolically less active vacuolar compartment exhibited a relaxation time comparable to that of the extracellular signal. 133Cs spectra of excised, maize root tips and excised top sections of the root adjacent to the kernel, each grown in 10 mM CsCl showed a difference in the relative areas of the Cs resonance corresponding to the distinct cytoplasm/vacuole volume ratio of these well differentiated sections of the root. The high correlation of counterion concentration with 133Cs chemical shifts suggested that the larger downfield shift exhibited by the cytoplasmic confined Cs+ was due principally to the higher ionic strength and protein content in this compartment. Such observations indicate that 133Cs-NMR might be employed for studying ionic strength, and osmotic pressure associated chemical shifts and the transport properties of Cs+ (perhaps as an analogue for K+) in subcellular compartments of plant tissues.

PMID: 2400781 [PubMed - indexed for MEDLINE]



Source: PubMed