Visualizing Proteins in Mammalian Cells by 19F NMR spectroscopy
 

Visualizing Proteins in Mammalian Cells by 19F NMR spectroscopy

In-cell NMR spectroscopy is a powerful tool to investigate protein behavior in physiologically relevant environments. Although proven valuable for disordered proteins, we show that in commonly used 1 H- 15 N HSQC spectra of globular proteins, interactions with cellular components often broaden resonances beyond detection. This contrasts 19 F spectra in mammalian cells, in which signals are readily observed. Using several proteins, we demonstrate that surface charges and interaction with cellular...

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