Very simple combination of TROSY, CRINEPT and multiple quantum coherence for signal enhancement in an HN(CO)CA experiment for large proteins
 

Very simple combination of TROSY, CRINEPT and multiple quantum coherence for signal enhancement in an HN(CO)CA experiment for large proteins


Publication year: 2011
Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 3 February 2011</br>
Monika, Bayrhuber , Roland, Riek</br>
Sensitivity enhancement in liquid state nuclear magnetic resonance (NMR) triple resonance experiments for the sequential assignment of proteins is important for the investigation of large proteins or protein complexes. We present here the 3D TROSY-MQ/CRINEPT-HN(CO)CA which makes use of a 15N-1H-TROSY element and a 13C’-13CA CRINEPT step combined with a multiple quantum coherence during the 13CA evolution period. Because of the introduction of these relaxation-optimized elements and ten less pulses required, when compared with the conventional TROSY-HN(CO)CA experiment an average signal enhancement of a factor of 1.8 was observed for the membrane protein-detergent complex KcsA with a rotational correlation time...</br>
Graphical abstract

*Graphical abstract:**Research highlights:*? 3D TROSY-MQ/CRINEPT-HN(CO)CA is a high sensitive NMR triple resonance experiment for sequential assignment ? A signal enhancement of a factor of ~1.8 is obtained on the membrane protein KcsA when compared with a conventional TROSY-based HN(CO)CA experiment
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Source: Journal of Magnetic Resonance