BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > Educational resources > Journal club
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 08-22-2010, 03:41 AM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 17,586
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default Validation of a noninvasive method to measure brain temperature in vivo using 1H NMR

Validation of a noninvasive method to measure brain temperature in vivo using 1H NMR spectroscopy.

Related Articles Validation of a noninvasive method to measure brain temperature in vivo using 1H NMR spectroscopy.

J Neurochem. 1995 Mar;64(3):1224-30

Authors: Corbett RJ, Laptook AR, Tollefsbol G, Kim B

The goal of this study was to evaluate the potential of using the difference between the 1H NMR frequencies of water and N-acetylaspartic acid (NAA) to measure brain temperature noninvasively. All water-suppressed and non-water-suppressed 1H NMR spectra were obtained at a field strength of 4.7 T using a surface coil. Experiments performed on model solutions revealed a decrease in the difference between NMR frequencies for NAA and water as a linear function of increasing temperature from 14 to 45 degrees C. Changing pH in the range 5.5-7.6 produced no discernible trends for concurrent changes in the slope and intercept of the linear relationship. There were minor changes in slope and intercept for solutions containing 80 or 100 mg of protein/ml versus no protein, but these changes were not considered to be of sufficient magnitude to deter the use of this approach to measure brain temperature. The protein content of swine cerebral cortex was found to remain constant from newborn to 1 month old (78 +/- 12 mg/g; n = 41). Therefore, data collected for the model solution containing 80 mg of protein/ml were used as a calibration curve to calculate brain temperature in eight swine during control, hypothermia, ischemia, postischemia, or death, over a temperature range of 23-40 degrees C. A plot of 61 temperatures determined from 1H NMR versus temperatures measured from an optical fiber probe sensor implanted 1 cm into the cerebral cortex showed excellent linear agreement (slope = 1.00 +/- 0.03, r2 = 0.96). We conclude that 1H NMR spectroscopy presents a practical means of making noninvasive measurements of brain temperature with an accuracy of better than +/- 1 degree C.

PMID: 7861155 [PubMed - indexed for MEDLINE]



Source: PubMed
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
In vivo oxygen-17 NMR for imaging brain oxygen metabolism at high field
In vivo oxygen-17 NMR for imaging brain oxygen metabolism at high field Publication year: 2011 Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 23 April 2011</br> Xiao-Hong, Zhu , Wei, Chen</br> *Highlights:*? This article reviews the developments of in vivo 17O NMR imaging in brain research. ? In vivo 17O NMR imaging has improved significantly at high/ultrahigh field. ? In vivo 17O NMR can noninvasively image brain oxygen metabolism and perfusion. ? In vivo 17O NMR is useful for mapping the functional change in oxygen...
nmrlearner Journal club 0 04-24-2011 03:42 PM
[NMRwiki tweet] nmrwiki: Is there a way to measure #chemical potential of polymer by #nmr? http://qa.
nmrwiki: Is there a way to measure #chemical potential of polymer by #nmr? http://qa.nmrwiki.org/question/173/ nmrwiki: Is there a way to measure #chemical potential of polymer by #nmr? http://qa.nmrwiki.org/question/173/ Source: NMRWiki tweets
nmrlearner Twitter NMR 0 09-15-2010 04:02 AM
Molecular design made to measure and the requirements - Nanotechwire.com
Molecular design made to measure and the requirements - Nanotechwire.com <img alt="" height="1" width="1" /> Molecular design made to measure and the requirements Nanotechwire.com NMR spectroscopy, a specialty of the "NMR Core Facility" at the University of Constance, provides an alternative and complementation to other methods. ... and more &raquo; Read here
nmrlearner Online News 0 08-23-2010 09:14 PM
[NMR paper] Application of 1H NMR chemical shifts to measure the quality of protein structures.
Application of 1H NMR chemical shifts to measure the quality of protein structures. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Application of 1H NMR chemical shifts to measure the quality of protein structures. J Mol Biol. 1995 Apr 7;247(4):541-6 Authors: Williamson MP, Kikuchi J, Asakura T We have developed a program that can calculate proton NMR chemical shifts for proteins, using a set of co-ordinates provided for example from an X-ray or NMR structure. When...
nmrlearner Journal club 0 08-22-2010 03:41 AM
[U. of Ottawa NMR Facility Blog] Temperature Calibration - An Alternative Method
Temperature Calibration - An Alternative Method It is well known that the actual temperature of a sample in an NMR probe is not necessarily the same as that read from the variable temperature unit on the spectrometer. This is because the thermocouple used by the variable temperature unit is below the sample tube and not in the center of the rf coil where the NMR measurements are made. One normally must make a calibration plot for the actual temperature vs. the set temperature. For temperatures above room temperature this can be done by employing the known temperature dependent chemical...
nmrlearner News from NMR blogs 0 08-21-2010 08:15 PM
[NMR tweet] Is it Possible to Extract Brain Metabolic Pathways Information from In Vivo H Nuclear
Is it Possible to Extract Brain Metabolic Pathways Information from In Vivo H Nuclear Magnetic Resonance Spectroscopy ? http://bit.ly/9fUMgB Published by PhysicsPaper (New Physics Papers) on 2010-08-17T02:45:59Z Source: Twitter
nmrlearner Twitter NMR 0 08-16-2010 11:59 PM
Molecular Design Made to Measure - AZoNano.com
Molecular Design Made to Measure - AZoNano.com <img alt="" height="1" width="1" /> Molecular Design Made to Measure AZoNano.com NMR spectroscopy, a specialty of the "NMR Core Facility" at the University of Constance, provides an alternative and complementation to other methods. ... and more &raquo; Read here
nmrlearner Online News 0 08-16-2010 03:50 AM
Why can't we simply use the earth's magnetic field to measure an NMR spectrum?
Why do we need to use large magnets and not simply the earth's magnetic field to measure an NMR spectrum?
Leon NMR Questions and Answers 2 12-03-2002 11:23 AM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2017, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 04:08 PM.


Map