Using 19F NMR to Probe Biological Interactions of Proteins and Peptides.
 

Using 19F NMR to Probe Biological Interactions of Proteins and Peptides.

Related Articles Using 19F NMR to Probe Biological Interactions of Proteins and Peptides.

ACS Chem Biol. 2014 Apr 24;

Authors: Marsh EN, Suzuki Y

Abstract
Fluorine is a valuable probe for investigating the interactions of biological molecules because of its favorable NMR characteristics, its small size and its near total absence from biology. Advances in biosynthetic methods allow fluorine to be introduced into peptides and proteins with high precision, and the increasing sensitivity of NMR spectrometers has facilitated the use of 19F NMR to obtain molecular-level insights into a wide range of often-complex biological interactions. Here we summarize the advantages of 19F NMR for studying the interactions of peptides and proteins, review methods for the production of fluorine-labeled materials, and describe some representative recent examples in which 19F NMR has been used to study conformational changes in peptides and proteins and their interactions with other biological molecules.


PMID: 24762032 [PubMed - as supplied by publisher]



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