Unlike twins: an NMR comparison of two ?-synuclein polymorphs featuring different toxicity.
 

Unlike twins: an NMR comparison of two ?-synuclein polymorphs featuring different toxicity.

http://www.bionmr.com//www.ncbi.nlm....one_120x30.png http://www.bionmr.com//www.ncbi.nlm....pubmed-pmc.gif Related Articles Unlike twins: an NMR comparison of two ?-synuclein polymorphs featuring different toxicity.

PLoS One. 2014;9(3):e90659

Authors: Gath J, Bousset L, Habenstein B, Melki R, Böckmann A, Meier BH

Abstract
We structurally compare, using solid-state NMR, two different polymorphs of ?-synuclein which, as established recently, display contrasting biochemical properties, toxicity, and tropism for cells. We show that both forms, which can each be produced as a pure polymorph, are greatly different in secondary structure. While ?-sheets are the dominating secondary structure elements for both polymorphs, they are markedly divergent in terms of number of elements, as well as their distribution. We demonstrate that all identified ?-sheets feature an in-register parallel stacking for both polymorphs. The two forms show a different molecular arrangement in the unit cell and distinct dynamic features, while sharing a highly flexible C-terminal domain. The use of reproducible, well-identified conditions for sample preparation and the recording of identical NMR experiments allows for a direct comparison of the results.


PMID: 24599158 [PubMed - indexed for MEDLINE]



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