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-   -   [NMR paper] Ultrashort Broadband Cooperative Pulses for Multidimensional Biomolecular NMR Experiments (http://www.bionmr.com/forum/journal-club-9/ultrashort-broadband-cooperative-pulses-multidimensional-biomolecular-nmr-experiments-25783/)

nmrlearner 03-07-2018 08:27 PM

Ultrashort Broadband Cooperative Pulses for Multidimensional Biomolecular NMR Experiments
 
Ultrashort Broadband Cooperative Pulses for Multidimensional Biomolecular NMR Experiments


NMR spectroscopy at ultra-high magnetic fields requires improved radiofrequency (rf) pulses to cover the increased spectral bandwidth. We introduce optimized 90° pulse pairs as Ramsey-type cooperative (Ram-COOP) pulses for biomolecular NMR applications. The Ram-COOP element provides broadband excitation with enhanced sensitivity and reduced artifacts even at magnetic fields >1.0 GHz 1H Larmor frequency (23 T). A pair of 30 ?s Ram-COOP pulses achieves an excitation bandwidth of 100 kHz with a maximum rf field of 20 kHz, more than three-fold improved compared to achievable excitation by rectangular pulses. Ram-COOP pulses exhibit little offset-dependent phase errors and are robust to rf inhomogeneity. The performance of the Ram-COOP element is experimentally confirmed with heteronuclear multidimensional NMR experiments, applied to proteins and nucleic acids. Ram-COOP provides broadband excitation at low rf field strength suitable for application at current magnetic fields and beyond 23 T.

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