In-cell NMR in E. coli to Monitor Maturation Steps of hSOD1.
PLoS One. 2011;6(8):e23561
Authors: Banci L, Barbieri L, Bertini I, Cantini F, Luchinat E
Abstract
In-cell NMR allows characterizing the folding state of a protein as well as posttranslational events at molecular level, in the cellular context. Here, the initial maturation steps of human copper, zinc superoxide dismutase 1 are characterized in the E. coli cytoplasm by in-cell NMR: from the apo protein, which is partially unfolded, to the zinc binding which causes its final quaternary structure. The protein selectively binds only one zinc ion, whereas in vitro also the copper site binds a non-physiological zinc ion. However, no intramolecular disulfide bridge formation occurs, nor copper uptake, suggesting the need of a specific chaperone for those purposes.
Nuclear Magnetic Resonance to characterize and monitor Cultural Heritage
Nuclear Magnetic Resonance to characterize and monitor Cultural Heritage
Publication year: 2011
Source: Progress in Nuclear Magnetic Resonance Spectroscopy, Available online 9 November 2011</br>
Donatella*Capitani, Valeria*Di Tullio, Noemi*Proietti</br>
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11-10-2011 07:38 AM
Long-Lived States to Monitor Protein Unfolding by Proton NMR.
Long-Lived States to Monitor Protein Unfolding by Proton NMR.
Long-Lived States to Monitor Protein Unfolding by Proton NMR.
Chemphyschem. 2011 Aug 31;
Authors: Bornet A, Ahuja P, Sarkar R, Fernandes L, Hadji S, Lee SY, Haririnia A, Fushman D, Bodenhausen G, Vasos PR
Abstract
The relaxation of long-lived states (LLS) corresponds to the slow return to statistical thermal equilibrium between symmetric and antisymmetric proton spin states. This process is remarkably sensitive to the presence of external spins and can be used to obtain...
Protein Interactions in the Escherichia coli Cytosol: An Impediment to In-Cell NMR Spectroscopy.
Protein Interactions in the Escherichia coli Cytosol: An Impediment to In-Cell NMR Spectroscopy.
Protein Interactions in the Escherichia coli Cytosol: An Impediment to In-Cell NMR Spectroscopy.
Chembiochem. 2011 Mar 29;
Authors: Crowley PB, Chow E, Papkovskaia T
Protein science is shifting towards experiments performed under native or native-like conditions. In-cell NMR spectroscopy for instance has the potential to reveal protein structure and dynamics inside cells. However, not all proteins can be studied by this technique. (15) N-labelled...
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03-31-2011 06:24 PM
[NMR paper] In-cell NMR spectroscopy.
In-cell NMR spectroscopy.
Related Articles In-cell NMR spectroscopy.
Methods Enzymol. 2005;394:17-41
Authors: Serber Z, Corsini L, Durst F, Dötsch V
The role of a protein inside a cell is determined by both its location and its conformational state. Although fluorescence techniques are widely used to determine the cellular localization of proteins in vivo, these approaches cannot provide detailed information about a protein's three-dimensional state. This gap, however, can be filled by NMR spectroscopy, which can be used to investigate both...
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11-24-2010 11:14 PM
[NMR paper] Optimization of an Escherichia coli system for cell-free synthesis of selectively N-l
Optimization of an Escherichia coli system for cell-free synthesis of selectively N-labelled proteins for rapid analysis by NMR spectroscopy.
Related Articles Optimization of an Escherichia coli system for cell-free synthesis of selectively N-labelled proteins for rapid analysis by NMR spectroscopy.
Eur J Biochem. 2004 Oct;271(20):4084-93
Authors: Ozawa K, Headlam MJ, Schaeffer PM, Henderson BR, Dixon NE, Otting G
Cell-free protein synthesis offers rapid access to proteins that are selectively labelled with amino acids and suitable for...
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11-24-2010 10:01 PM
NMR methods to monitor the enzymatic depolymerization of heparin.
NMR methods to monitor the enzymatic depolymerization of heparin.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles NMR methods to monitor the enzymatic depolymerization of heparin.
Anal Bioanal Chem. 2010 Sep 4;
Authors: Limtiaco JF, Beni S, Jones CJ, Langeslay DJ, Larive CK
Heparin and the related glycosaminoglycan, heparan sulfate, are polydisperse linear polysaccharides that mediate numerous biological processes due to their interaction with proteins....
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09-05-2010 05:53 AM
Dissecting the Microscopic Steps of the Cyclophilin A Enzymatic Cycle on the biologic
Dissecting the Microscopic Steps of the Cyclophilin A Enzymatic Cycle on the biological substrate HIV-capsid by NMR.
Related Articles Dissecting the Microscopic Steps of the Cyclophilin A Enzymatic Cycle on the biological substrate HIV-capsid by NMR.
J Mol Biol. 2010 Aug 11;
Authors: Bosco DA, Eisenmesser EZ, Clarkson MW, Wolf-Watz M, Labeikovsky W, Millet O, Kern D
Peptidyl-prolyl isomerases (PPIases) are emerging as key regulators of many diverse biological processes. Elucidating the role of PPIase activity in vivo has been challenging because...
In-cell NMR in E. coli to Monitor Maturation Steps of hSOD1.
Linear Mode
