NMR paper Two-dimensional 1H and 15N NMR titration studies of hisactophilin.

		BioNMR > Educational resources > Journal club
[NMR paper] Two-dimensional 1H and 15N NMR titration studies of hisactophilin.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

View First Unread

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-17-2010, 11:06 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,184

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Two-dimensional 1H and 15N NMR titration studies of hisactophilin.

Two-dimensional 1H and 15N NMR titration studies of hisactophilin.

Related Articles Two-dimensional 1H and 15N NMR titration studies of hisactophilin.

Biochem Cell Biol. 1998;76(2-3):294-301

Authors: Hammond MS, Houliston RS, Meiering EM

We have used two-dimensional 1H-15N heteronuclear single quantum correlation spectroscopy to measure the pH dependence of backbone amide group chemical shifts in the actin binding protein hisactophilin over the pH range 5.7-11.1. Most of the resonances can be analyzed using a simple equation involving a single apparent ionization constant, pK(app). The majority of resonances in the protein titrate with pK(app) values of 5.6-7.4. The results can be rationalized in terms of titration of many histidine residues in hisactophilin. The titration data provide direct experimental support for the proposed models of the atomic basis of actin and membrane binding by hisactophilin.

PMID: 9923698 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Supramolecular interactions between losartan and hydroxypropyl-?-CD: ESI mass-spectrometry, NMR techniques, phase solubility, isothermal titration calorimetry and anti-hypertensive studies. Supramolecular interactions between losartan and hydroxypropyl-?-CD: ESI mass-spectrometry, NMR techniques, phase solubility, isothermal titration calorimetry and anti-hypertensive studies. Supramolecular interactions between losartan and hydroxypropyl-?-CD: ESI mass-spectrometry, NMR techniques, phase solubility, isothermal titration calorimetry and anti-hypertensive studies. Int J Pharm. 2011 Feb 14;404(1-2):116-23 Authors: de Paula WX, Denadai AM, Santoro MM, Braga AN, Santos RA, Sinisterra RD In this work, low soluble supramolecular complex...	nmrlearner	Journal club	0	04-13-2011 11:57 PM
[NMR paper] NMR studies and redox titration of the tetraheme cytochrome c3 from Desulfomicrobium NMR studies and redox titration of the tetraheme cytochrome c3 from Desulfomicrobium baculatum. Identification of the low-potential heme. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles NMR studies and redox titration of the tetraheme cytochrome c3 from Desulfomicrobium baculatum. Identification of the low-potential heme. Eur J Biochem. 1995 Jun 15;230(3):1007-13 Authors: Coutinho IB, Turner DL, Legall J, Xavier AV The tetraheme...	nmrlearner	Journal club	0	08-22-2010 03:41 AM
[NMR paper] Two-dimensional NMR studies of selenomethionyl calmodulin. Two-dimensional NMR studies of selenomethionyl calmodulin. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Two-dimensional NMR studies of selenomethionyl calmodulin. J Mol Biol. 1994 Jun 17;239(4):545-54 Authors: Zhang M, Vogel HJ Calmodulin (CaM) is a ubiquitous calcium regulatory protein that can interact with almost 30 different target proteins. The majority of the CaM-binding domains of the target proteins are believed to interact with two hydrophobic surfaces on...	nmrlearner	Journal club	0	08-22-2010 03:33 AM
[NMR paper] Two-dimensional NMR studies of selenomethionyl calmodulin. Two-dimensional NMR studies of selenomethionyl calmodulin. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Two-dimensional NMR studies of selenomethionyl calmodulin. J Mol Biol. 1994 Jun 17;239(4):545-54 Authors: Zhang M, Vogel HJ Calmodulin (CaM) is a ubiquitous calcium regulatory protein that can interact with almost 30 different target proteins. The majority of the CaM-binding domains of the target proteins are believed to interact with two hydrophobic surfaces on...	nmrlearner	Journal club	0	08-22-2010 03:33 AM
[NMR paper] Two- and three-dimensional proton NMR studies of apo-neocarzinostatin. Two- and three-dimensional proton NMR studies of apo-neocarzinostatin. Related Articles Two- and three-dimensional proton NMR studies of apo-neocarzinostatin. Biochemistry. 1991 Aug 6;30(31):7730-9 Authors: Gao XL, Burkhart W Neocarzinostatin (NCS) is an antitumor protein from Streptomyces carzinostaticus that is identical in apo-protein sequence with mitomalcin (MMC) from Streptomyces malayensis. We describe the use of apo-NCS as a model system for applying combined two- and three-dimensional (2D and 3D) proton NMR spectroscopy to the...	nmrlearner	Journal club	0	08-21-2010 11:12 PM
[NMR paper] Two- and three-dimensional proton NMR studies of apo-neocarzinostatin. Two- and three-dimensional proton NMR studies of apo-neocarzinostatin. Related Articles Two- and three-dimensional proton NMR studies of apo-neocarzinostatin. Biochemistry. 1991 Aug 6;30(31):7730-9 Authors: Gao XL, Burkhart W Neocarzinostatin (NCS) is an antitumor protein from Streptomyces carzinostaticus that is identical in apo-protein sequence with mitomalcin (MMC) from Streptomyces malayensis. We describe the use of apo-NCS as a model system for applying combined two- and three-dimensional (2D and 3D) proton NMR spectroscopy to the...	nmrlearner	Journal club	0	08-21-2010 11:12 PM
[NMR paper] Two-dimensional 1H-NMR studies of horseradish peroxidase C and its interaction with i Two-dimensional 1H-NMR studies of horseradish peroxidase C and its interaction with indole-3-propionic acid. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Two-dimensional 1H-NMR studies of horseradish peroxidase C and its interaction with indole-3-propionic acid. Eur J Biochem. 1990 Apr 30;189(2):351-62 Authors: Veitch NC, Williams RJ The binding of aromatic donor molecules to plant peroxidases has been investigated by examining...	nmrlearner	Journal club	0	08-21-2010 10:48 PM
[NMR paper] One- and two-dimensional NMR studies of the N-terminal portion of glycophorin A at 11 One- and two-dimensional NMR studies of the N-terminal portion of glycophorin A at 11.7 Tesla. Related Articles One- and two-dimensional NMR studies of the N-terminal portion of glycophorin A at 11.7 Tesla. J Protein Chem. 1990 Apr;9(2):129-36 Authors: Dill K, Hu SH, Berman E, Pavia AA, Lacombe JM One- and two-dimensional nuclear magnetic resonance (NMR) spectroscopy (at 11.7 Tesla) was used to gain some structural and spectral information about glycophorin AM, glycophorin AM tryptic glycopeptide, a related pentapeptide, and two related...	nmrlearner	Journal club	0	08-21-2010 10:48 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off