NMR paper Tubulin-tyrosine ligase catalyzes covalent binding of 3-fluoro-tyrosine to tubulin: k

		BioNMR > Educational resources > Journal club
[NMR paper] Tubulin-tyrosine ligase catalyzes covalent binding of 3-fluoro-tyrosine to tubulin: k

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

View First Unread

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-22-2010, 03:50 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,174

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Tubulin-tyrosine ligase catalyzes covalent binding of 3-fluoro-tyrosine to tubulin: k

Tubulin-tyrosine ligase catalyzes covalent binding of 3-fluoro-tyrosine to tubulin: kinetic and [19F]NMR studies.

Related Articles Tubulin-tyrosine ligase catalyzes covalent binding of 3-fluoro-tyrosine to tubulin: kinetic and [19F]NMR studies.

FEBS Lett. 1995 Oct 30;374(2):165-8

Authors: Monasterio O, Nova E, LÃ³pez-Brauet A, Lagos R

The use of 3-fluoro-tyrosine as an alternative substrate for the enzyme tubulin:tyrosine ligase which catalyzes the incorporation of tyrosine into the alpha-tubulin subunit was investigated. The incorporation of tyrosine into tubulin was inhibited competitively by 3-fluoro-tyrosine with an apparent Ki of approximately 25 microM. The affinity for this analog was similar to that of tyrosine, confirming that the hydrogen at position 3 of the aromatic ring is not essential for the reaction catalyzed by TTLase. The incorporation of 3-fluoro-tyrosine into the C-terminus of the alpha-tubulin subunit was demonstrated through [19F]NMR spectroscopy. The 3-fluoro-tyrosine signal at -58.6 ppm (trifluoroacetic acid as external standard), with a bandwidth of 24.7 Hz presented a chemical shift of 0.75 ppm upfield and an enlargement in the bandwidth (30.5 Hz) when incorporated into tubulin. These results strongly suggest that this amino acid is exposed to the solvent in tubulin. Tubulin covalently labeled with 3-fluoro-tyrosine was competent to polymerize into microtubules. The use of fluorinated tubulin in [19F]NMR spectroscopy for studying questions concerning protein conformation and interactions will be discussed.

PMID: 7589526 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[Question from NMRWiki Q&A forum] unusual tyrosine cross peaks unusual tyrosine cross peaks Hi I am observing some unexpected cross peaks in some homonuclear protein data and I was wanting to know if anyone had an ideas what im looking at. I am looking at what I was confident was a tyrosine HE-HD cross peak in a TOCSY giving charachterticaly intense cross peaks at 6.9 x 6.4 ppm, however I then noticed a second set of TOCSY peaks to both the HE* and HD* at 5.4 ppm which also gives a COSY to the HE. These peaks shift together if I change the temperature sugesting it is not just two overlaped resonances, are still present in D2O, both the HE and HD* give...	nmrlearner	News from other NMR forums	0	10-17-2011 08:40 AM
Insights into the interaction of discodermolide and docetaxel with tubulin. Mapping the binding sites of microtubule-stabilizing agents by using an integrated NMR and computational approach. Insights into the interaction of discodermolide and docetaxel with tubulin. Mapping the binding sites of microtubule-stabilizing agents by using an integrated NMR and computational approach. Insights into the interaction of discodermolide and docetaxel with tubulin. Mapping the binding sites of microtubule-stabilizing agents by using an integrated NMR and computational approach. ACS Chem Biol. 2011 May 3; Authors: Canales A, R Salarichs J, Trigili C, Nieto L, Coderch C, Andreu JM, Paterson I, Jimenez-Barbero J, Díaz Pereira JF The binding...	nmrlearner	Journal club	0	05-06-2011 02:00 AM
[NMR paper] NMR studies of the RRsrc peptide, a tyrosine kinase substrate. NMR studies of the RRsrc peptide, a tyrosine kinase substrate. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.nrc-cnrc.gc.ca-cisti-journals-rp-gifs-PubMed_logo_e.gif Related Articles NMR studies of the RRsrc peptide, a tyrosine kinase substrate. Biochem Cell Biol. 1997;75(2):163-9 Authors: Brockbank RL, Vogel HJ The proton and carbon-13 NMR resonances for the 13-residue synthetic RRsrc peptide were completely assigned using two-dimensional NMR spectroscopy. This peptide contains a tyrosine in position 9 that can be phosphorylated...	nmrlearner	Journal club	0	08-22-2010 03:31 PM
[NMR paper] NMR studies of the RRsrc peptide, a tyrosine kinase substrate. NMR studies of the RRsrc peptide, a tyrosine kinase substrate. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.nrc-cnrc.gc.ca-cisti-journals-rp-gifs-PubMed_logo_e.gif Related Articles NMR studies of the RRsrc peptide, a tyrosine kinase substrate. Biochem Cell Biol. 1997;75(2):163-9 Authors: Brockbank RL, Vogel HJ The proton and carbon-13 NMR resonances for the 13-residue synthetic RRsrc peptide were completely assigned using two-dimensional NMR spectroscopy. This peptide contains a tyrosine in position 9 that can be phosphorylated...	nmrlearner	Journal club	0	08-22-2010 03:03 PM
[NMR paper] The solution conformation of tubulin-beta(422-434)-NH2 and its Nac-DATADEQG-NH2 fragm The solution conformation of tubulin-beta(422-434)-NH2 and its Nac-DATADEQG-NH2 fragment based on NMR. Related Articles The solution conformation of tubulin-beta(422-434)-NH2 and its Nac-DATADEQG-NH2 fragment based on NMR. Biopolymers. 1991 Mar;31(4):449-58 Authors: Otter A, Scott PG, Maccioni RB, Kotovych G The solution conformation of tubulin-beta(422-434)-NH2 (YQQYQDATADEQG-NH2) and its Nac-DATADEQG-NH2 fragment has been studied by two-dimensional 1H-nmr spectroscopy in CD3OH/H2O (90/10 v/v) at neutral and low pH. The 13 amino acid peptide...	nmrlearner	Journal club	0	08-21-2010 11:16 PM
[NMR paper] The interaction of [13C]-enriched colchicine with tubulin as determined by NMR spectr The interaction of -enriched colchicine with tubulin as determined by NMR spectroscopy. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles The interaction of -enriched colchicine with tubulin as determined by NMR spectroscopy. Biochim Biophys Acta. 1991 Jul 12;1078(3):339-44 Authors: Osei AA, Everett GW, Ringel I, Himes RH Colchicine, labeled at the tropolone ring methoxy carbon, was used to study interactions with tubulin containing either Mg2+ or Mn2+ at the high...	nmrlearner	Journal club	0	08-21-2010 11:12 PM
[NMR paper] The interaction of [13C]-enriched colchicine with tubulin as determined by NMR spectr The interaction of -enriched colchicine with tubulin as determined by NMR spectroscopy. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles The interaction of -enriched colchicine with tubulin as determined by NMR spectroscopy. Biochim Biophys Acta. 1991 Jul 12;1078(3):339-44 Authors: Osei AA, Everett GW, Ringel I, Himes RH Colchicine, labeled at the tropolone ring methoxy carbon, was used to study interactions with tubulin containing either Mg2+ or Mn2+ at the high...	nmrlearner	Journal club	0	08-21-2010 11:12 PM
[NMR paper] NMR structure of phospho-tyrosine signaling complexes. NMR structure of phospho-tyrosine signaling complexes. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_120x27.gif Related Articles NMR structure of phospho-tyrosine signaling complexes. Med Res Rev. 1999 Jul;19(4):295-305 Authors: Post CB, Gaul BS, Eisenmesser EZ, Schneider ML A structural basis for activation and substrate specificity of src tyrosine kinases, and regulation of protein-protein association by tyrosine phosphorylation is described. Lyn, a...	nmrlearner	Journal club	0	08-21-2010 04:03 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off