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Default TROSY-selected ZZ-exchange experiment for characterizing slow chemical exchange in large proteins

TROSY-selected ZZ-exchange experiment for characterizing slow chemical exchange in large proteins


Abstract A TROSY-selected ZZ-exchange experiment is described for measuring slow chemical exchange rates by monitoring the TROSY component of 15N longitudinal magnetization. Application of the proposed pulse sequence to the cadherin 8 N-terminal extracelluar domain demonstrates that enhanced sensitivity is obtained, compared to a previously described TROSY-detected ZZ-exchange sequence (Sahu et al. J Am Chem Soc 129: 13232â??13237, 2007), by preserving the TROSY effect during the mixing period as well as the frequency encoding periods.
  • Content Type Journal Article
  • Pages 357-360
  • DOI 10.1007/s10858-009-9385-0
  • Authors
    • Ying Li, Columbia University Department of Biochemistry and Molecular Biophysics 630 West 168th Street New York NY 10032 USA
    • Arthur G. Palmer, Columbia University Department of Biochemistry and Molecular Biophysics 630 West 168th Street New York NY 10032 USA

Source: Journal of Biomolecular NMR
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