Trimethylsilyl reporter groups for NMR studies of conformational changes in G protein-coupled receptors.
Trimethylsilyl reporter groups for NMR studies of conformational changes in G protein-coupled receptors.
Related Articles Trimethylsilyl reporter groups for NMR studies of conformational changes in G protein-coupled receptors. FEBS Lett. 2019 Apr 05;: Authors: Hu W, Wang H, Hou Y, Hao Y, Liu D Abstract Large membrane proteins such as G Protein-Coupled Receptors (GPCRs) are difficult for NMR study due to severe signal overlaps and unfavorable relaxation properties. We used a trimethylsilyl (TMS) group as a reporter group for 1 H NMR study of conformational changes in proteins, utilizing high-intensity 1 H NMR signals near 0 ppm. The ?2 -Adrenergic Receptor (?2 AR) was labeled with TMS groups at two cysteines located at the cytoplasmic ends of helices VI and VII. Binding of various ligands led to changes in 1 H NMR signals, which manifested that helix VI is sensitive to G protein-specific activation, whereas helix VII is sensitive to ?-arrestin-specific activation. Thus, the TMS group is a useful reporter group in NMR for studying conformational changes in membrane proteins such as GPCRs. This article is protected by copyright. All rights reserved. PMID: 30953343 [PubMed - as supplied by publisher] More... |
All times are GMT. The time now is 08:39 PM. |
Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Search Engine Friendly URLs by vBSEO 3.6.0
Copyright, BioNMR.com, 2003-2013