The transient expression of recombinant proteins in plant cell packs facilitates stable isotope labeling for NMR spectroscopy
 

The transient expression of recombinant proteins in plant cell packs facilitates stable isotope labeling for NMR spectroscopy

Nuclear magnetic resonance (NMR) spectroscopy can be used to determine the structure, dynamics and interactions of proteins. However, protein NMR requires stable isotope labeling for signal detection. The cells used for the production of recombinant proteins must therefore be grown in medium containing isotopically labeled substrates. Stable isotope labeling is well established in Escherichia coli, but bacteria are only suitable for the production of simple proteins without post-translational...

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