Time-resolved DEER EPR and solid-state NMR afford kinetic and structural elucidation of substrate binding to Ca2+-ligated calmodulin [Chemistry]
 

Time-resolved DEER EPR and solid-state NMR afford kinetic and structural elucidation of substrate binding to Ca2+-ligated calmodulin [Chemistry]

Thomas Schmidt, Jaekyun Jeon, Wai-Ming Yau, Charles D. Schwieters, Robert Tycko, G. Marius Clore...
Date: 2022-02-01

Recent advances in rapid mixing and freeze quenching have opened the path for time-resolved electron paramagnetic resonance (EPR)-based double electron-electron resonance (DEER) and solid-state NMR of protein–substrate interactions. DEER, in conjunction with phase memory time filtering to quantitatively extract species populations, permits monitoring time-dependent probability distance distributions between pairs of... Read More


PNAS:
Number: 6
Volume: 119