The three dimensional structure of rat cytokine CINC/Gro in solution by homonuclear 3
 

The three dimensional structure of rat cytokine CINC/Gro in solution by homonuclear 3D NMR.

http://www.ncbi.nlm.nih.gov/corehtml...PubMedLink.gif Related Articles The three dimensional structure of rat cytokine CINC/Gro in solution by homonuclear 3D NMR.

FEBS Lett. 1994 Nov 7;354(2):207-12

Authors: Hanzawa H, Haruyama H, Watanabe K, Tsurufuji S

The solution conformation of rat cytokine-induced neutrophil chemoattractant (CINC/Gro), a small protein consisting of 72 amino acid residues with proinflammatory activities, and a member of the interleukin 8 family corresponding to a counterpart of human Gro, was investigated with homonuclear 2D and 3D NMR spectroscopy. At each phase of the structural analysis, the homonuclear 3D NOESY-HOHAHA and HOHAHA-NOESY spectra afforded valuable data, removing ambiguities intractable by conventional 2D NMR techniques. CINC/Gro exists as a dimer in solution and contains a triple stranded anti-parallel beta-sheet and C-terminal alpha-helix in the monomer structure, as observed in human IL-8, but non-trivial differences are also observed.

PMID: 7957925 [PubMed - indexed for MEDLINE]



Source: PubMed