A thermosensitive gel matrix for bioreactor-assisted in-cell NMR of nucleic acids and proteins
A thermosensitive gel matrix for bioreactor-assisted in-cell NMR of nucleic acids and proteins
Introducing the flow through the bioreactor has revolutionized in-cell NMR spectroscopy by prolonging the measurement time available to acquire spectral information about biomacromolecules in metabolically active cells. Bioreactor technology relies on immobilizer matrices, which secure cells in the active volume of the NMR coil and enable uniform perfusion of the growth medium, supplying fresh nutrients to the cells while removing toxic byproducts of their metabolism. The main drawbacks of... More... |
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