Related ArticlesA theoretical assessment of structure determination of multi-span membrane proteins by oriented sample solid-state NMR spectroscopy.
Aust J Chem. 2020;73(3):246-251
Authors: Weber DK, Veglia G
Abstract
Oriented sample solid state NMR (OS-ssNMR) spectroscopy allows direct determination of the structure and topology of membrane proteins reconstituted into aligned lipid bilayers. While OS-ssNMR theoretically has no upper size limit, its application to multi-span membrane proteins has not been established since most studies have been restricted to single or dual span proteins and peptides. Here, we present a critical assessment of the application of this method to multi-span membrane proteins. We used molecular dynamics simulations to back-calculate [15N-1H] separated local field (SLF) spectra from a G protein-coupled receptor (GPCR) and show that fully resolved spectra can be obtained theoretically for a multi-span membrane protein with currently achievable resonance linewidths.
[NMR paper] Macrodiscs Comprising SMALPs for Oriented Sample Solid-State NMR Spectroscopy of Membrane Proteins.
Macrodiscs Comprising SMALPs for Oriented Sample Solid-State NMR Spectroscopy of Membrane Proteins.
Related Articles Macrodiscs Comprising SMALPs for Oriented Sample Solid-State NMR Spectroscopy of Membrane Proteins.
Biophys J. 2018 Jun 15;:
Authors: Radoicic J, Park SH, Opella SJ
Abstract
Macrodiscs, which are magnetically alignable lipid bilayer discs with diameters of >30*nm, were obtained by solubilizing protein-containing liposomes with styrene-maleic acid copolymers. Macrodiscs provide a detergent-free phospholipid bilayer...
nmrlearner
Journal club
0
06-20-2018 08:56 PM
Macrodiscs Comprising SMALPs for Oriented Sample Solid-State NMR Spectroscopy of Membrane Proteins
Macrodiscs Comprising SMALPs for Oriented Sample Solid-State NMR Spectroscopy of Membrane Proteins
Publication date: Available online 15 June 2018
Source:Biophysical Journal</br>
Author(s): Jasmina Radoicic, Sang Ho Park, Stanley J. Opella</br>
Macrodiscs, which are magnetically alignable lipid bilayer discs with diameters of >30*nm, were obtained by solubilizing protein-containing liposomes with styrene-maleic acid copolymers. Macrodiscs provide a detergent-free phospholipid bilayer environment for biophysical and functional studies of membrane proteins under...
nmrlearner
Journal club
0
06-16-2018 07:20 AM
[NMR paper] Membrane Protein Structural Validation by Oriented Sample Solid-State NMR: Diacylglycerol Kinase.
Membrane Protein Structural Validation by Oriented Sample Solid-State NMR: Diacylglycerol Kinase.
Related Articles Membrane Protein Structural Validation by Oriented Sample Solid-State NMR: Diacylglycerol Kinase.
Biophys J. 2014 Apr 15;106(8):1559-69
Authors: Murray DT, Li C, Gao FP, Qin H, Cross TA
Abstract
The validation of protein structures through functional assays has been the norm for many years. Functional assays perform this validation for water-soluble proteins very well, but they need to be performed in the same...
nmrlearner
Journal club
0
04-18-2014 01:35 PM
Membrane Protein Structural Validation by Oriented Sample Solid-State NMR: Diacylglycerol Kinase
Membrane Protein Structural Validation by Oriented Sample Solid-State NMR: Diacylglycerol Kinase
Publication date: 15 April 2014
Source:Biophysical Journal, Volume 106, Issue 8</br>
Author(s): Dylan*T. Murray , Conggang Li , F.*Philip Gao , Huajun Qin , Timothy*A. Cross</br>
The validation of protein structures through functional assays has been the norm for many years. Functional assays perform this validation for water-soluble proteins very well, but they need to be performed in the same environment as that used for the structural analysis. This is difficult...
[NMR paper] Resonance assignments of a membrane protein in phospholipid bilayers by combining multiple strategies of oriented sample solid-state NMR.
Resonance assignments of a membrane protein in phospholipid bilayers by combining multiple strategies of oriented sample solid-state NMR.
Related Articles Resonance assignments of a membrane protein in phospholipid bilayers by combining multiple strategies of oriented sample solid-state NMR.
J Biomol NMR. 2013 Dec 20;
Authors: Lu GJ, Opella SJ
Abstract
Oriented sample solid-state NMR spectroscopy can be used to determine the three-dimensional structures of membrane proteins in magnetically or mechanically aligned lipid bilayers. The...
A theoretical assessment of structure determination of multi-span membrane proteins by oriented sample solid-state NMR spectroscopy.
Linear Mode
