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Default Techniques and applications of NMR to membrane proteins.

Techniques and applications of NMR to membrane proteins.

Related Articles Techniques and applications of NMR to membrane proteins.

Mol Membr Biol. 2004 May-Jun;21(3):129-41

Authors: Nielsen N, Malmendal A, Vosegaard T

The fact that membrane proteins are notoriously difficult to analyse using standard protocols for atomic-resolution structure determination methods have motivated adaptation of these techniques to membrane protein studies as well as development of new technologies. With this motivation, liquid-state nuclear magnetic resonance (NMR) has recently been used with success for studies of peptides and membrane proteins in detergent micelles, and solid-state NMR has undergone a tremendous evolution towards characterization of membrane proteins in native membrane and oriented phospholipid bilayers. In this mini-review, we describe some of the technological challenges behind these efforts and provide examples on their use in membrane biology.

PMID: 15204621 [PubMed - indexed for MEDLINE]



Source: PubMed
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