Targeted preparation and NMR spectroscopic characterization of Lys11-linked ubiquitin trimers
 

Targeted preparation and NMR spectroscopic characterization of Lys11-linked ubiquitin trimers

Ubiquitylation refers to the attachment of mono- or poly-ubiquitin molecules to a substrate protein. To shield ubiquitin chains against potential hydrolysis, a facile, click-chemistry based approach was recently established for the generation of site-specifically conjugated ubiquitin dimers relying on triazole linkage. Here, the preparation of such ubiquitin chains was advanced by the generation of homotypic Lys11-linked ubiquitin trimers considering an isotopic labeling scheme in a moiety-wise...

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