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-   -   [NMR paper] Studying the assembly of the BAM complex in native membranes by cellular solid-state NMR spectroscopy. (http://www.bionmr.com/forum/journal-club-9/studying-assembly-bam-complex-native-membranes-cellular-solid-state-nmr-spectroscopy-25393/)

nmrlearner 12-05-2017 07:35 PM

Studying the assembly of the BAM complex in native membranes by cellular solid-state NMR spectroscopy.
 
Studying the assembly of the BAM complex in native membranes by cellular solid-state NMR spectroscopy.

Studying the assembly of the BAM complex in native membranes by cellular solid-state NMR spectroscopy.

J Struct Biol. 2017 Nov 29;:

Authors: Pinto C, Mance D, Julien M, Daniels M, Weingarth M, Baldus M

Abstract
Significant progress has been made in obtaining a structural insight into the assembly of the ?-barrel assembly machinery complex (BAM). These crystallography and electron microscopy studies used detergent as a membrane mimetic and revealed structural variations in the central domain, BamA, as well as the lipoprotein BamC. We have used cellular solid-state NMR spectroscopy to examine the entire BamABCDE complex in native outer membranes and obtained data on the BamCDE subcomplex in outer membranes, in addition to synthetic bilayers. To reduce spectral crowding, we utilized proton-detected experiments and employed amino-acid specific isotope-labelling in (13C, 13C) correlation experiments. Taken together, the results provide insight into the overall fold and assembly of the BAM complex in native membranes, in particular regarding the structural flexibility of BamC in the absence of the core unit BamA.


PMID: 29197585 [PubMed - as supplied by publisher]



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