NMR paper Studies on phosphorylated transcriptional regulator (NarL) for E. coli nar operon by

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[NMR paper] Studies on phosphorylated transcriptional regulator (NarL) for E. coli nar operon by

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NMR processing:

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Side-chains:

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Structure from NMR restraints:

Ab initio:

Fragment-based:

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Camcoil

Poulsen_rc_CS

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08-22-2010, 03:01 AM

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Studies on phosphorylated transcriptional regulator (NarL) for E. coli nar operon by

Studies on phosphorylated transcriptional regulator (NarL) for E. coli nar operon by 31P-NMR spectroscopy.

Related Articles Studies on phosphorylated transcriptional regulator (NarL) for E. coli nar operon by 31P-NMR spectroscopy.

Biochem Mol Biol Int. 1993 Sep;31(1):161-8

Authors: Takahashi K, Hattori T, Shindo H, Noji S, Nohno T, Taniguchi S

The sequential transphosphorylation from autophosphorylated nitrate-sensing protein (NarX) to the transcriptional regulator protein (NarL), both operating in signal transduction to control the expression of the respiratory nitrate reductase (nar) operon in E. coli, was demonstrated with an in vitro reconstructed system to function similarly to other bacterial two-component regulatory systems. Over-expression system established by means of the pT7 promoter/polymerase provided both NarX and NarL proteins to reconstruct the in vitro transphosphorylation system. The phosphorylated NarL was detected, and the unstable phosphorylated group was directly assigned to acyl phosphate in the in vitro system by 31P-NMR spectroscopy.

PMID: 8260940 [PubMed - indexed for MEDLINE]

Source: PubMed

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