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-   -   [NMR paper] Structural studies of biomaterials using double-quantum solid-state NMR spectroscopy. (http://www.bionmr.com/forum/journal-club-9/structural-studies-biomaterials-using-double-quantum-solid-state-nmr-spectroscopy-9746/)

nmrlearner 11-24-2010 08:49 PM

Structural studies of biomaterials using double-quantum solid-state NMR spectroscopy.
 
Structural studies of biomaterials using double-quantum solid-state NMR spectroscopy.

Related Articles Structural studies of biomaterials using double-quantum solid-state NMR spectroscopy.

Annu Rev Phys Chem. 2003;54:531-71

Authors: Drobny GP, Long JR, Karlsson T, Shaw W, Popham J, Oyler N, Bower P, Stringer J, Gregory D, Mehta M, Stayton PS

Proteins directly control the nucleation and growth of biominerals, but the details of molecular recognition at the protein-biomineral interface remain poorly understood. The elucidation of recognition mechanisms at this interface may provide design principles for advanced materials development in medical and ceramic composites technologies. Here, we describe both the theory and practice of double-quantum solid-state NMR (ssNMR) structure-determination techniques, as they are used to determine the secondary structures of surface-adsorbed peptides and proteins. In particular, we have used ssNMR dipolar techniques to provide the first high-resolution structural and dynamic characterization of a hydrated biomineralization protein, salivary statherin, adsorbed to its biologically relevant hydroxyapatite (HAP) surface. Here, we also review NMR data on peptides designed to adsorb from aqueous solutions onto highly porous hydrophobic surfaces with specific helical secondary structures. The adsorption or covalent attachment of biological macromolecules onto polymer materials to improve their biocompatibility has been pursued using a variety of approaches, but key to understanding their efficacy is the verification of the structure and dynamics of the immobilized biomolecules using double-quantum ssNMR spectroscopy.

PMID: 12709513 [PubMed - indexed for MEDLINE]



Source: PubMed


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