Structural and dynamic studies of DNA recognition by NF-?B p50 RHR homodimer using methyl NMR spectroscopy
 

Structural and dynamic studies of DNA recognition by NF-?B p50 RHR homodimer using methyl NMR spectroscopy

Protein dynamics involving higher-energy sparsely populated conformational substates are frequently critical for protein function. This study describes the dynamics of the homodimer (p50)2 of the p50 Rel homology region (RHR) of the transcription factor NF-?B, using 13C relaxation dispersion experiments with specifically (13C, 1H)-labeled methyl groups of Ile (?), Leu and Val. Free (p50)2 is highly dynamic in solution, showing ?s-ms relaxation dispersion consistent with exchange between the...

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