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Default NMR Chemical Shift Mapping of SH2 Peptide Interactions.

NMR Chemical Shift Mapping of SH2 Peptide Interactions.

Related Articles NMR Chemical Shift Mapping of SH2 Peptide Interactions.

Methods Mol Biol. 2017;1555:269-290

Authors: McKercher MA, Wuttke DS

Abstract
Heteronuclear single quantum coherence (HSQC) nuclear magnetic resonance (NMR) experiments offer a rapid and high resolution approach to gaining binding and conformational insights into a protein-peptide interaction. By tracking (1)H and (15)N chemical shift changes over the course of a peptide titration into isotopically labeled protein, amide NH pairs of amino acids whose chemical environment changes upon peptide binding can be identified. When mapped onto a structure of the protein, this approach can identify the peptide-binding interface or regions undergoing conformation changes within a protein upon ligand binding. Monitoring NMR chemical shift changes can also serve as a screening technique to identify novel interaction partners for a protein or to determine the binding affinity of a weak protein-peptide interaction. Here, we describe the application of NMR chemical shift mapping to the study of peptide binding to the C-terminal SH2 domain of PLC?1.


PMID: 28092038 [PubMed - in process]



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