Strategies for solid-state NMR investigations of supramolecular assemblies with large subunit sizes
 

Strategies for solid-state NMR investigations of supramolecular assemblies with large subunit sizes

Publication date: Available online 15 November 2014
Source:Journal of Magnetic Resonance</br>
Author(s): Pascal Fricke , Veniamin Chevelkov , Chaowei Shi , Adam Lange</br>
Solid-state NMR is a versatile tool to study structure and dynamics of insoluble and non-crystalline biopolymers. Supramolecular protein assemblies are formed by self-association of multiple copies of single small-sized proteins. Because of their high degree of local order, solid-state NMR spectra of such systems exhibit an unusually high level of resolution, rendering them an ideal target for solid-state NMR investigations. Recently, our group has solved the structure of one particular supramolecular assembly, the type-iii-secretion-system needle. The needle subunit comprises around 80 residues. Many interesting supramolecular assemblies with unknown structure have subunits larger in size, which requires development of tailored solid-state NMR strategies to address their structures. In this “Perspective” article, we provide a view on different approaches to enhance sensitivity and resolution in biological solid-state NMR with a focus on the possible application to supramolecular assemblies with large subunit sizes.
Graphical abstract

http://origin-ars.els-cdn.com/conten...002985-fx1.jpg</br></br>
</br></br>

More...