NMR paper A straight-forward method of optimising protein solubility for NMR.

		BioNMR > Educational resources > Journal club
[NMR paper] A straight-forward method of optimising protein solubility for NMR.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

View First Unread

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-24-2010, 10:03 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,184

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

A straight-forward method of optimising protein solubility for NMR.

A straight-forward method of optimising protein solubility for NMR.

Related Articles A straight-forward method of optimising protein solubility for NMR.

J Biomol NMR. 2004 Nov;30(3):283-6

Authors: Howe PW

Maximising solubility is a key step in applying solution-state NMR to proteins. The 'microbatch' crystallisation screening method can be adapted to screen for protein solubility. In this approach, drops of test solutions are placed under paraffin oil in 96-well screening plates. This requires very small amounts of protein, is easy to set up and is readily automatable.

PMID: 15756459 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Improving protein solubility & long-term stability Has anybody successfully used the following method? A simple method for improving protein solubility and long-term stability. Golovanov AP, Hautbergue GM, Wilson SA, Lian LY. Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology, P. O. Box 88, Manchester M60 1QD, UK. a.golovanov@umist.ac.jp J Am Chem Soc. 2004 Jul 28;126(29):8933-9.	nmrlearner	Proteins	4	01-22-2014 07:04 PM
[Question from NMRWiki Q&A forum] optimising o1 in solid state nmr optimising o1 in solid state nmr I'm a little bit confused about setting o1, in the case of a solid sample First o1 is the offset of the transmitter frequency so sfo1 = BF1 + o1In my case I have to set Sfo1 = BF1 so I must set o1 = 0 HzSecond there is another way to optimize o1 whish is by applying gs and looking the fid, adjusting o1 until we have a good exponential decrease of the fid. In this case o1 value may not be 0 Hz.Does the second technique is valid in solid stat experiences?And which one I have to use? Check if somebody has answered this question on NMRWiki QA forum	nmrlearner	News from other NMR forums	0	03-09-2011 04:19 AM
[Question from NMRWiki Q&A forum] optimising o1 in solid stat nmr optimising o1 in solid stat nmr I'm a little bit confused about setting o1, in the case of a solid sample First o1 is the offset of the transmitter frequency so sfo1 = BF1 + o1In my case I have to set Sfo1 = BF1 so I must set o1 = 0 HzSecond there is another way to optimize o1 whish is by applying gs and looking the fid, adjusting o1 until we have a good exponential decrease of the fid. In this case o1 value may not be 0 Hz.Does the second technique is valid in solid stat experiences?And which one I have to use? Check if somebody has answered this question on NMRWiki QA forum	nmrlearner	News from other NMR forums	0	03-08-2011 04:10 PM
Overcoming the solubility limit with solubility-enhancement tags: successful applications in biomolecular NMR studies Overcoming the solubility limit with solubility-enhancement tags: successful applications in biomolecular NMR studies Abstract Although the rapid progress of NMR technology has significantly expanded the range of NMR-trackable systems, preparation of NMR-suitable samples that are highly soluble and stable remains a bottleneck for studies of many biological systems. The application of solubility-enhancement tags (SETs) has been highly effective in overcoming solubility and sample stability issues and has enabled structural studies of important biological systems previously deemed...	nmrlearner	Journal club	0	01-09-2011 12:46 PM
Celebrating its 20th anniversary in 2011, the Journal of Biomolecular NMR looks forward to the second decade of the 21st century Celebrating its 20th anniversary in 2011, the Journal of Biomolecular NMR looks forward to the second decade of the 21st century Celebrating its 20th anniversary in 2011, the Journal of Biomolecular NMR looks forward to the second decade of the 21st century Content Type Journal Article DOI 10.1007/s10858-010-9466-0 Authors	nmrlearner	Journal club	0	12-31-2010 08:38 PM
[NMR tweet] IM SO OVER THIS: and not looking forward to 2moro 4hour nuclear magnetic resonance sp IM SO OVER THIS: and not looking forward to 2moro 4hour nuclear magnetic resonance spectroscopy prac; like fml. Published by iloveitt26 (millie/.! ) on 2010-09-12T10:43:34Z Source: Twitter	nmrlearner	Twitter NMR	0	09-12-2010 11:10 AM
NMR - Keep It Straight NMR - Keep It Straight http://i.ytimg.com/vi/33jBum3Uu-k/default.jpg NMR - Keep It Straight Tallinn Hardcore From: euronoor Views: 11494 http://gdata.youtube.com/static/images/icn_star_full_11x11.gif http://gdata.youtube.com/static/images/icn_star_full_11x11.gif http://gdata.youtube.com/static/images/icn_star_full_11x11.gif http://gdata.youtube.com/static/images/icn_star_full_11x11.gif http://gdata.youtube.com/static/images/icn_star_half_11x11.gif 44 ratings	nmrlearner	NMR educational videos	0	08-18-2010 01:38 AM
Optimisation of protein solubility for NMR A straight-forward method of optimising protein solubility for NMR. Howe PW. Analytical Sciences, Syngenta, Jealott's Hill Research Centre, Bracknell, Berkshire, RG42 6EY, UK. http://public.metapress.com/images/covers/publications/102922.jpg J Biomol NMR. 2004 Nov;30(3):283-6 Abstract: Maximising solubility is a key step in applying solution-state NMR to proteins. The 'microbatch' crystallisation screening method can be adapted to screen for protein solubility. In this approach, drops of test solutions are placed under paraffin oil in 96-well screening plates. This requires very...	nmrlearner	Proteins	0	08-14-2005 07:53 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off