Stable isotope labeling methods for DNA
 

Stable isotope labeling methods for DNA


Publication date: Available online 20 June 2016
Source:Progress in Nuclear Magnetic Resonance Spectroscopy</br>
Author(s): Frank H.T. Nelissen, Marco Tessari, Sybren S. Wijmenga, Hans A. Heus</br>
NMR is a powerful method for studying proteins and nucleic acids in solution. The study of nucleic acids by NMR is far more challenging than for proteins, which is mainly due to the limited number of building blocks and unfavorable spectral properties. For NMR studies of DNA molecules, (site specific) isotope enrichment is required to facilitate specific NMR experiments and applications. Here, we provide a comprehensive review of isotope-labeling strategies for obtaining stable isotope labeled DNA as well as specifically stable isotope labeled building blocks required for enzymatic DNA synthesis.
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