Sparsely-sampled High-resolution 4-D Experiments for Efficient Backbone Resonance Assignment of Disordered Proteins
Sparsely-sampled High-resolution 4-D Experiments for Efficient Backbone Resonance Assignment of Disordered Proteins
Publication year: 2011 Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 4 January 2011</br> Jie, Wen , Jihui, Wu , Pei, Zhou</br> Intrinsically disordered proteins (IDPs) play important roles in many critical cellular processes. Due to their limited chemical shift dispersion, IDPs often require four pairs of resonance connectivities (H?, C?, C? and CO) for establishing sequential backbone assignment. Because most conventional 4-D triple-resonance experiments share an overlapping C? evolution period, combining existing 4-D experiments does not offer an optimal solution for non-redundant collection of a complete set of backbone resonances. Using alternative chemical shift evolution schemes, we propose a new pair of 4-D triple resonance experiments—HA(CA)CO(CA)NH/ HA(CA)CONH—that complement the 4-D HNCACB/ HN(CO)CACB experiments to provide complete backbone resonance information. Collection of...</br> http://www.sciencedirect.com/cache/M...JX-51W05BV-1-2</br></br> Source: Journal of Magnetic Resonance |
All times are GMT. The time now is 11:51 AM. |
Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Search Engine Friendly URLs by vBSEO 3.6.0
Copyright, BioNMR.com, 2003-2013