Solution structures of human transforming growth factor alpha derived from 1H NMR dat
 

Solution structures of human transforming growth factor alpha derived from 1H NMR data.

Related Articles Solution structures of human transforming growth factor alpha derived from 1H NMR data.

Biochemistry. 1990 Aug 28;29(34):7805-13

Authors: Kline TP, Brown FK, Brown SC, Jeffs PW, Kopple KD, Mueller L

The 600-MHz 1H NMR spectrum of the des-Val-Val mutant of human transforming growth factor alpha (TGF-alpha) was reassigned at pH = 6.3. The conformation space of des-Val-Val TGF-alpha was explored by distance geometry embedding followed by restrained molecular dynamics refinement using NOE distance constraints and some torsion angle constraints derived from J-couplings. Over 80 long-range NOE constraints were found by completely assigning all resolved cross-peaks in the NOESY spectra. Low NOE constraint violations were observed in structures obtained with the following three different refinement procedures: interactive annealing in DSPACE, AMBER 3.0 restrained molecular dynamics, and dynamic simulated annealing in XPLOR. The segment from Phe15 to Asp47 was found to be conformationally well-defined. Back-calculations of NOESY spectra were used to evaluate the quality of the structures. Our calculated structures resemble the ribbon diagram presentations that were recently reported by other groups. Several side-chain conformations appear to be well-defined as does the relative orientation of the C loop to the N-terminal half of the protein.

PMID: 2261437 [PubMed - indexed for MEDLINE]



Source: PubMed