A solution NMR toolset to probe the molecular mechanisms of amyloid inhibitors.
 

A solution NMR toolset to probe the molecular mechanisms of amyloid inhibitors.

http://www.bionmr.com//www.ncbi.nlm...._z_RSClogo.gif Related Articles A solution NMR toolset to probe the molecular mechanisms of amyloid inhibitors.

Chem Commun (Camb). 2018 May 03;54(37):4644-4652

Authors: Ahmed R, Melacini G

Abstract
The self-association of the amyloid beta (A?) peptide into toxic oligomers is implicated in the early events leading to Alzheimer's disease (AD). Blocking the formation of A? oligomers and their interactions with the extracellular and cellular environment through small molecules and biopharmaceuticals is therefore a promising preventive strategy for AD. However, given the heterogeneity and transient nature of the A? oligomeric species, detailed structural and kinetic characterizations of such oligomers and oligomer:inhibitor complexes have proven to be challenging. Here, we discuss recent advancements in solution NMR that have been instrumental in overcoming these limitations and we provide two representative examples of A? inhibitors from our work to demonstrate the applications of such experiments, i.e. EGCG and human serum albumin.


PMID: 29658548 [PubMed - indexed for MEDLINE]



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