Solution NMR Spectroscopy of Supra-Molecular Systems, Why Bother? A Methyl TROSY View
 

Solution NMR Spectroscopy of Supra-Molecular Systems, Why Bother? A Methyl TROSY View


Publication year: 2011
Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 8 March 2011</br>
Lewis E., Kay</br>
With the development of appropriate labeling schemes and the associated experiments that exploit them it has become possible to record high quality solution NMR spectra of supra-molecular complexes with molecular masses extending to 1MDa. One such approach involves selective 13CH3 methyl labeling in highly deuterated proteins using experiments that make use of a methyl-TROSY effect that significantly improves both resolution and sensitivity in spectra. The utility of this methodology has been demonstrated on a growing number of interesting particles. It seems appropriate at this juncture, therefore, to ‘step back’ and evaluate the role that solution NMR spectroscopy can play in...</br>
Graphical abstract

*Graphical abstract:*Studies of MDa complexes by solution-state NMR using Methyl-TROSY: An application to the proteasome.*Highlights:*? Qualitative explanation of how 1H-13C and 1H-1H dipolar cross-correlated relaxation in a 13CH3 group attached to a macromolecule can be exploited for studies of supra-molecular structure, dynamics and function. Studies of the proteasome (670 kDa) via methyl-TROSY NMR emphasize the importance of dynamics to the function of this large complex. Studies of proteasome substrates show that they are maintained in a disordered state inside the antechamber.
http://www.sciencedirect.com/cache/M...JX-52BGCWX-5-2</br></br>

Source: Journal of Magnetic Resonance