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nmrlearner 08-22-2010 03:31 PM

Solid-state REDOR NMR distance measurements at the ligand site of a bacterial chemota
 
Solid-state REDOR NMR distance measurements at the ligand site of a bacterial chemotaxis membrane receptor.

http://www.ncbi.nlm.nih.gov/corehtml...es-acspubs.jpg Related Articles Solid-state REDOR NMR distance measurements at the ligand site of a bacterial chemotaxis membrane receptor.

Biochemistry. 1997 Feb 18;36(7):1699-703

Authors: Wang J, Balazs YS, Thompson LK

The Escherichia coli serine receptor senses serine levels in the environment and transmits this information across the bacterial inner membrane to modulate a protein phosphorylation cascade which controls swimming behavior. Solid-state nuclear magnetic resonance (NMR) has been used to characterize specific structural features of the ligand binding site interactions in the intact, membrane-bound Ser receptor. Rotational-echo double-resonance (REDOR) experiments on [15N]Ser bound to a [1-13C]Phe-receptor preparation are used to measure distances between the ligand amino group and the carbonyls of two phenylalanine residues in the ligand binding pocket. The results indicate two 4.0 +/- 0.2 A distances, in excellent agreement with the X-ray crystal structure of a soluble fragment of the homologous aspartate receptor [Milburn et al. (1991) Science 254, 1342-1347]. These results confirm the similarity of the binding sites of the Asp and Ser receptors, and demonstrate the feasibility of using solid-state NMR measurements to obtain specific structural information on the 120 kDa intact receptor for probing transmembrane signaling mechanisms.

PMID: 9048553 [PubMed - indexed for MEDLINE]



Source: PubMed


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