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Default Solid-state NMR sequential assignments of the N-terminal domain of HpDnaB helicase.

Solid-state NMR sequential assignments of the N-terminal domain of HpDnaB helicase.

Related Articles Solid-state NMR sequential assignments of the N-terminal domain of HpDnaB helicase.

Biomol NMR Assign. 2015 Aug 18;

Authors: Wiegand T, Gardiennet C, Ravotti F, Bazin A, Kunert B, Lacabanne D, Cadalbert R, Güntert P, Terradot L, Böckmann A, Meier BH

Abstract
We present solid-state NMR assignments of the N-terminal domain of the DnaB helicase from Helicobacter pylori (153 residues) in its microcrystalline form. We use a sequential resonance assignment strategy based on three-dimensional NMR experiments. The resonance assignments obtained are compared with automated resonance assignments computed with the ssFLYA algorithm. An analysis of the (13)C secondary chemical shifts determines the position of the secondary structure elements in this ?-helical protein.


PMID: 26280528 [PubMed - as supplied by publisher]



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