Solid-state NMR and membrane proteins.
 

Solid-state NMR and membrane proteins.

Related Articles Solid-state NMR and membrane proteins.

J Magn Reson. 2014 Dec 29;

Authors: Opella SJ

Abstract
The native environment for a membrane protein is a phospholipid bilayer. Because the protein is immobilized on NMR timescales by the interactions within a bilayer membrane, solid-state NMR methods are essential to obtain high-resolution spectra. Approaches have been developed for both unoriented and oriented samples, however, they all rest on the foundation of the most fundamental aspects of solid-state NMR, and the chemical shift and homo- and hetero-nuclear dipole-dipole interactions. Solid-state NMR has advanced sufficiently to enable the structures of membrane proteins to be determined under near-native conditions in phospholipid bilayers.


PMID: 25681966 [PubMed - as supplied by publisher]



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