We have prepared mouse fur extensively 13C,15N-labelled in all amino acid types enabling application of 2D solid state NMR techniques which establish covalent and spatial proximities within, and in favorable cases between, residues. 13C double quantumâ??single quantum correlation and proton driven spin diffusion techniques are particularly useful for resolving certain amino acid types. Unlike 1D experiments on isotopically normal material, the 2D methods allow the chemical shifts of entire spin systems of numerous residue types to be determined, particularly those with one or more distinctively shifted atoms such as Gly, Ser, Thr, Tyr, Phe, Val, Leu, Ile and Pro. Also the partial resolution of the amide signals into two signal envelopes comprising of α-helical, and β-sheet/random coil components, enables resolution of otherwise overlapped α-carbon signals into two distinct cross peak families corresponding to these respective secondary structural regions. The increase in resolution conferred by extensive labelling offers new opportunities to study the chemical fate and structural environments of specific atom and amino acid types under the influence of commercial processes, and therapeutic or cosmetic treatments.
Correlating Synthetic Methods, Morphology, Atomic-Level Structure, and Catalytic Activity of Sn-? Catalysts
From The DNP-NMR Blog:
Correlating Synthetic Methods, Morphology, Atomic-Level Structure, and Catalytic Activity of Sn-? Catalysts
Wolf, P., et al., Correlating Synthetic Methods, Morphology, Atomic-Level Structure, and Catalytic Activity of Sn-? Catalysts. ACS Catalysis, 2016. 6(7): p. 4047-4063.
http://dx.doi.org/10.1021/acscatal.6b00114
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07-13-2016 10:22 PM
[NMR paper] Atomic-resolution structure of cytoskeletal bactofilin by solid-state NMR.
Atomic-resolution structure of cytoskeletal bactofilin by solid-state NMR.
Atomic-resolution structure of cytoskeletal bactofilin by solid-state NMR.
Sci Adv. 2015 Dec;1(11):e1501087
Authors: Shi C, Fricke P, Lin L, Chevelkov V, Wegstroth M, Giller K, Becker S, Thanbichler M, Lange A
Abstract
Bactofilins are a recently discovered class of cytoskeletal proteins of which no atomic-resolution structure has been reported thus far. The bacterial cytoskeleton plays an essential role in a wide range of processes, including...
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12-15-2015 08:09 PM
[NMR paper] The contribution of solid-state NMR spectroscopy to understanding biomineralization: Atomic and molecular structure of bone.
The contribution of solid-state NMR spectroscopy to understanding biomineralization: Atomic and molecular structure of bone.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif The contribution of solid-state NMR spectroscopy to understanding biomineralization: Atomic and molecular structure of bone.
J Magn Reson. 2015 Apr;253:98-110
Authors: Duer MJ
Abstract
Solid-state NMR spectroscopy has had a major impact on our understanding of the structure of...
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03-24-2015 09:58 PM
[NMR paper] The contribution of solid-state NMR spectroscopy to understanding biomineralization: Atomic and molecular structure of bone
The contribution of solid-state NMR spectroscopy to understanding biomineralization: Atomic and molecular structure of bone
Publication date: April 2015
Source:Journal of Magnetic Resonance, Volume 253</br>
Author(s): Melinda J. Duer</br>
Solid-state NMR spectroscopy has had a major impact on our understanding of the structure of mineralized tissues, in particular bone. Bone exemplifies the organic–inorganic composite structure inherent in mineralized tissues. The organic component of the extracellular matrix in bone is primarily composed of ordered fibrils of...
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03-22-2015 06:36 PM
[NMR paper] Cationic membrane peptides: atomic-level insight of structure-activity relationships from solid-state NMR.
Cationic membrane peptides: atomic-level insight of structure-activity relationships from solid-state NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Cationic membrane peptides: atomic-level insight of structure-activity relationships from solid-state NMR.
Amino Acids. 2013 Mar;44(3):821-33
Authors: Su Y, Li S, Hong M
Abstract
Many membrane-active peptides, such as cationic cell-penetrating peptides (CPPs) and antimicrobial peptides...
Molecular-Level Examination of Cu2+ Binding Structure for Amyloid Fibrils of 40-Residue Alzheimer’s ? by Solid-State NMR Spectroscopy
Molecular-Level Examination of Cu2+ Binding Structure for Amyloid Fibrils of 40-Residue Alzheimer’s ? by Solid-State NMR Spectroscopy
Sudhakar Parthasarathy, Fei Long, Yifat Miller, Yiling Xiao, Dan McElheny, Kent Thurber, Buyong Ma, Ruth Nussinov and Yoshitaka Ishii
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja1072178/aop/images/medium/ja-2010-072178_0006.gif
Journal of the American Chemical Society
DOI: 10.1021/ja1072178
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA ...
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02-22-2011 11:06 PM
Atomic-resolution three-dimensional structure of HET-s(218-289) amyloid fibrils by solid-state NMR spectroscopy.
Atomic-resolution three-dimensional structure of HET-s(218-289) amyloid fibrils by solid-state NMR spectroscopy.
Atomic-resolution three-dimensional structure of HET-s(218-289) amyloid fibrils by solid-state NMR spectroscopy.
J Am Chem Soc. 2010 Oct 6;132(39):13765-75
Authors: Van Melckebeke H, Wasmer C, Lange A, Ab E, Loquet A, Böckmann A, Meier BH
We present a strategy to solve the high-resolution structure of amyloid fibrils by solid-state NMR and use it to determine the atomic-resolution structure of the prion domain of the fungal prion HET-s...
Solid state NMR of isotope labelled murine fur: a powerful tool to study atomic level keratin structure and treatment effects
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