Site-specific protein backbone deuterium 2H? quadrupolar patterns by proton-detected quadruple-resonance 3D 2H?c?NH MAS NMR spectroscopy
 

Site-specific protein backbone deuterium 2H? quadrupolar patterns by proton-detected quadruple-resonance 3D 2H?c?NH MAS NMR spectroscopy

A novel deuterium-excited and proton-detected quadruple-resonance three-dimensional (3D) ²H(?)c(?)NH MAS nuclear magnetic resonance (NMR) method is presented to obtain site-specific ²H(?) deuterium quadrupolar couplings from protein backbone, as an extension to the 2D version of the experiment reported earlier. Proton-detection results in high sensitivity compared to the heteronuclei detection methods. Utilizing four independent radiofrequency (RF) channels (quadruple-resonance), we managed to...

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