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Default Simultaneous acquisition of PAR and PAIN spectra

Simultaneous acquisition of PAR and PAIN spectra


Abstract We present a scheme that allows the simultaneous detection of PAR and PAIN correlation spectra in a single two-dimensional experiment. For both spectra, we obtain almost the same signal-to-noise ratio as if a PAR or PAIN spectrum is recorded separately, which in turn implies that one of the spectra may be considered additional information for free. The experiment is based on the observation that in a PAIN experiment, the PAR condition is always also fulfilled. The performance is demonstrated experimentally using uniformly 13C,15N-labeled samples of Nâ??fâ??MLFâ??OH and ubiquitin.

  • Content Type Journal Article
  • Category Communication
  • Pages 1-6
  • DOI 10.1007/s10858-012-9616-7
  • Authors
    • Anders B. Nielsen, Physical Chemistry, ETH Zürich, Wolfgang-Pauli-Strasse 10, 8093 Zürich, Switzerland
    • Kathrin Székely, Physical Chemistry, ETH Zürich, Wolfgang-Pauli-Strasse 10, 8093 Zürich, Switzerland
    • Julia Gath, Physical Chemistry, ETH Zürich, Wolfgang-Pauli-Strasse 10, 8093 Zürich, Switzerland
    • Matthias Ernst, Physical Chemistry, ETH Zürich, Wolfgang-Pauli-Strasse 10, 8093 Zürich, Switzerland
    • Niels Chr. Nielsen, Interdisciplinary Nanoscience Center (iNANO) and Department of Chemistry, Center for Insoluble Protein Structures (inSPIN), University of Aarhus, 8000 Aarhus C, Denmark
    • Beat H. Meier, Physical Chemistry, ETH Zürich, Wolfgang-Pauli-Strasse 10, 8093 Zürich, Switzerland


Source: Journal of Biomolecular NMR
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