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Default Signal enhancement in protein NMR using the spin-noise tuning optimum

Signal enhancement in protein NMR using the spin-noise tuning optimum


Abstract We have assessed the potential of an alternative probe tuning strategy based on the spin-noise response for application in common high-resolution multi-dimensional biomolecular NMR experiments with water signal suppression on aqueous and salty samples. The method requires the adjustment of the optimal tuning condition, which may be offset by several 100 kHz from the conventional tuning settings using the noise response of the water protons as an indicator. Although the radio frequency-pulse durations are typically longer under such conditions, signal-to-noise gains of up to 22% were achieved. At salt concentrations up to 100 mM a substantial sensitivity gain was observed.

  • Content Type Journal Article
  • DOI 10.1007/s10858-010-9446-4
  • Authors
    • Martin Nausner, Institute of Organic Chemistry, Johannes Kepler University, AltenbergerstraÃ?e 69, 4040 Linz, Austria
    • Michael Goger, New York Structural Biology Center, 89 Convent Avenue, New York, NY 10027-7556, USA
    • Eli Bendet-Taicher, Chemistry Department, New York University, New York, NY 10003, USA
    • Judith Schlagnitweit, Institute of Organic Chemistry, Johannes Kepler University, AltenbergerstraÃ?e 69, 4040 Linz, Austria
    • Alexej Jerschow, Chemistry Department, New York University, New York, NY 10003, USA
    • Norbert Müller, Institute of Organic Chemistry, Johannes Kepler University, AltenbergerstraÃ?e 69, 4040 Linz, Austria


Source: Journal of Biomolecular NMR
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