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Default Rational tuning of fluorobenzene probes for cysteine-selective protein modification

Rational tuning of fluorobenzene probes for cysteine-selective protein modification


Fluorobenzene probes for protein profiling through selective cysteine labeling have been developed by rational reactivity tuning. Tuning was achieved by selecting an electron-withdrawing para-substituent combined with variation of the number of fluorine substituents. Optimized probes chemo-selectively arylated cysteine residues in proteins under aqueous conditions. Probes linked to azide, biotin or a fluorophore were applicable to labeling of eGFP and albumin. Selective inhibition of cysteine proteases was also demonstrated with the probes. Additionally, probes were tuned for site selective labeling among cysteine residues and for activity based protein profiling in cell lysates.

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