Sequential HNCACB and CBCANH protein NMR pulse sequences.
 

Sequential HNCACB and CBCANH protein NMR pulse sequences.

Related Articles Sequential HNCACB and CBCANH protein NMR pulse sequences.

J Magn Reson. 2001 Aug;151(2):328-31

Authors: Meissner A, Sørensen OW

The pulse sequences HNCACB and CBCANH correlating side chain C(beta) resonances with amide resonances in the protein backbone do not distinguish between inter- and intraresidue correlations. The new pulse sequences sequential HNCACB and sequential CBCANH make this distinction by suppressing coherence transfer between 13C(alpha) and 15N via the one-bond J(NC(alpha)) coupling so that only the sequential correlations are observed in the spectrum. The experimental results of applying sequential HNCACB in a clean-TROSY-adapted implementation to the protein Chymotrypsin Inhibitor 2 at 800 MHz are presented.

PMID: 11531355 [PubMed - indexed for MEDLINE]



Source: PubMed