Related ArticlesSedNMR: a web tool for optimizing sedimentation of macromolecular solutes for SSNMR.
J Biomol NMR. 2013 Dec;57(4):319-26
Authors: Ferella L, Luchinat C, Ravera E, Rosato A
Abstract
We have proposed solid state NMR (SSNMR) of sedimented solutes as a novel approach to sample preparation for biomolecular SSNMR without crystallization or other sample manipulations. The biomolecules are confined by high gravity--obtained by centrifugal forces either directly in a SSNMR rotor or in a ultracentrifugal device--into a hydrated non-crystalline solid suitable for SSNMR investigations. When gravity is removed, the sample reverts to solution and can be treated as any solution NMR sample. We here describe a simple web tool to calculate the relevant parameters for the success of the experiment.
SedNMR: On the Edge between Solution and Solid-State NMR
From The DNP-NMR Blog:
SedNMR: On the Edge between Solution and Solid-State NMR
Sedimented samples (states) can be described as a "microcrystaline glass", which provide a new approach for the preparation of DNP samples. This was described in a post this year you can find here (http://blog.bridge12.com/2013/02/dynamic-nuclear-polarization-of.html).
The following article gives more details about the approach.
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12-24-2013 12:59 AM
Dynamic Nuclear Polarization of Sedimented Solutes
From the The DNP-NMR Blog:
Dynamic Nuclear Polarization of Sedimented Solutes
Ravera, E., et al., Dynamic Nuclear Polarization of Sedimented Solutes. J. Am. Chem. Soc., 2013. 135(5): p. 1641-1644.
http://dx.doi.org/10.1021/ja312553b
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04-15-2013 08:52 AM
[NMR paper] Optimizing ssNMR experiments for dilute proteins in heterogeneous mixtures at high magnetic fields.
Optimizing ssNMR experiments for dilute proteins in heterogeneous mixtures at high magnetic fields.
Related Articles Optimizing ssNMR experiments for dilute proteins in heterogeneous mixtures at high magnetic fields.
Magn Reson Chem. 2007 Dec;45 Suppl 1:S209-20
Authors: McNeill SA, Gor'kov PL, Struppe J, Brey WW, Long JR
Abstract
Solid-state NMR spectroscopy at high magnetic fields is proving to be an effective technique in structural biology, particularly for proteins which are not amenable to traditional X-ray and solution NMR...
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04-03-2013 08:22 PM
[NMR paper] SedNMR: On the Edge between Solution and Solid-State NMR.
SedNMR: On the Edge between Solution and Solid-State NMR.
Related Articles SedNMR: On the Edge between Solution and Solid-State NMR.
Acc Chem Res. 2013 Mar 7;
Authors: Bertini I, Luchinat C, Parigi G, Ravera E
Abstract
Solid-state NMR (SS-NMR) of proteins requires that those molecules be immobilized, usually by crystallization, freezing, or lyophilization. However, self-crowding can also slow molecular rotation sufficiently to prevent the nuclear interactions from averaging. To achieve self-crowding, researchers can use a centrifugal field...
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03-09-2013 11:05 AM
Macromolecular NMR spectroscopy for the non-spectroscopist: beyond macromolecular solution structure determination.
Macromolecular NMR spectroscopy for the non-spectroscopist: beyond macromolecular solution structure determination.
Macromolecular NMR spectroscopy for the non-spectroscopist: beyond macromolecular solution structure determination.
FEBS J. 2011 Jan 7;
Authors: Bieri M, Kwan AH, Mobli M, King GF, Mackay JP, Gooley PR
A strength of NMR spectroscopy is its ability to monitor, on an atomic level, molecular changes and interactions. In this article, which is intended for non-spectroscopists, we describe major uses of NMR in protein science beyond...
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01-11-2011 11:27 PM
[NMR paper] Protein stabilization by compatible solutes. Effect of diglycerol phosphate on the dy
Protein stabilization by compatible solutes. Effect of diglycerol phosphate on the dynamics of Desulfovibrio gigas rubredoxin studied by NMR.
Related Articles Protein stabilization by compatible solutes. Effect of diglycerol phosphate on the dynamics of Desulfovibrio gigas rubredoxin studied by NMR.
Eur J Biochem. 2003 Dec;270(23):4606-14
Authors: Lamosa P, Turner DL, Ventura R, Maycock C, Santos H
Heteronuclear NMR relaxation measurements and hydrogen exchange data have been used to characterize protein dynamics in the presence or absence of...
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11-24-2010 09:16 PM
[NMR paper] Optimizing resolution in multidimensional NMR by three-way decomposition.
Optimizing resolution in multidimensional NMR by three-way decomposition.
Related Articles Optimizing resolution in multidimensional NMR by three-way decomposition.
J Biomol NMR. 2003 Oct;27(2):165-73
Authors: Orekhov VY, Ibraghimov I, Billeter M
Resolution depends on the number of points sampled in a FID; in indirectly detected dimensions it is an important determinant of the total experiment time. Based on the high redundancy present in NMR data, we propose the following timesaving scheme for three-dimensional spectra. An extensive grid of...
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11-24-2010 09:16 PM
[NMR paper] Transmembrane 19F NMR chemical shift difference of fluorinated solutes in liposomes,
Transmembrane 19F NMR chemical shift difference of fluorinated solutes in liposomes, erythrocytes and erythrocyte ghosts.
Related Articles Transmembrane 19F NMR chemical shift difference of fluorinated solutes in liposomes, erythrocytes and erythrocyte ghosts.
NMR Biomed. 1993 Mar-Apr;6(2):136-43
Authors: Xu AS, Waldeck AR, Kuchel PW
In erythrocytes suspended in isotonic medium, a number of fluorinated anions showed well resolved 19F NMR resonances from the solute populations in the intra- and extracellular compartments; the intracellular...
SedNMR: a web tool for optimizing sedimentation of macromolecular solutes for SSNMR.
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