BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > Educational resources > Journal club
Advanced Search



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Unread 10-02-2021, 06:00 AM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 21,849
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default Rigid, highly reactive and stable DOTA-like tags containing a thiol-specific phenylsulfonyl pyridine moiety for protein modification and NMR analysis

Rigid, highly reactive and stable DOTA-like tags containing a thiol-specific phenylsulfonyl pyridine moiety for protein modification and NMR analysis

Site specific labeling of paramagnetic NMR tags generate valuable structural restraints such as pseudocontact shifts (PCSs) and residual dipolar couplings (RDCs) that can be used to characterize structures, interactions and dynamics of protein-ligand or nucleic acid complexes. Herein, two single-armed DOTA-like tags, BrPSPy-DO3M( S )A-Ln and BrPSPy-6M-DO3M( S )A-Ln, each containing a thiol-specific reacting group, phenylsulfonyl pyridine moiety, are demonstrated as rigid, reactive and stable...

More...
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
Highly Stable Magic Angle Spinning Spherical Rotors Lacking Turbine Grooves
From The DNP-NMR Blog: Highly Stable Magic Angle Spinning Spherical Rotors Lacking Turbine Grooves Osborn Popp, Thomas M., Alexander Däpp, Chukun Gao, Pin-Hui Chen, Lauren E. Price, Nicholas H. Alaniva, and Alexander B. Barnes. “Highly Stable Magic Angle Spinning Spherical Rotors Lacking Turbine Grooves.” Preprint. Solid-state NMR/Instrumentation, April 1, 2020. https://doi.org/10.5194/mr-2020-2.
nmrlearner News from NMR blogs 0 08-07-2020 08:10 PM
[NMR paper] Quaternization of Vinyl/Alkynyl Pyridine Enables Ultrafast Cysteine-Selective Protein Modification and Charge Modulation
Quaternization of Vinyl/Alkynyl Pyridine Enables Ultrafast Cysteine-Selective Protein Modification and Charge Modulation Angewandte Chemie International Edition, EarlyView. More...
nmrlearner Journal club 0 04-09-2019 11:33 PM
[NMR paper] Stable and rigid DTPA-like paramagnetic tags suitable for in vitro and in situ protein NMR analysis.
Stable and rigid DTPA-like paramagnetic tags suitable for in vitro and in situ protein NMR analysis. Stable and rigid DTPA-like paramagnetic tags suitable for in vitro and in situ protein NMR analysis. J Biomol NMR. 2017 Dec 09;: Authors: Chen JL, Zhao Y, Gong YJ, Pan BB, Wang X, Su XC Abstract Organic synthesis of a ligand with high binding affinities for paramagnetic lanthanide ions is an effective way of generating paramagnetic effects on proteins. These paramagnetic effects manifested in high-resolution NMR spectroscopy are...
nmrlearner Journal club 0 12-11-2017 12:45 PM
Stable and rigid DTPA-like paramagnetic tags suitable for in vitro and in situ protein NMR analysis
Stable and rigid DTPA-like paramagnetic tags suitable for in vitro and in situ protein NMR analysis Abstract Organic synthesis of a ligand with high binding affinities for paramagnetic lanthanide ions is an effective way of generating paramagnetic effects on proteins. These paramagnetic effects manifested in high-resolution NMR spectroscopy are valuable dynamic and structural restraints of proteins and proteinâ??ligand complexes. A paramagnetic tag generally contains a metal chelating moiety and a reactive group for protein modification. Herein we...
nmrlearner Journal club 0 12-10-2017 01:21 AM
[NMR paper] Site-specific tagging proteins with a rigid, small and stable transition metal chelator, 8-hydroxyquinoline, for paramagnetic NMR analysis.
Site-specific tagging proteins with a rigid, small and stable transition metal chelator, 8-hydroxyquinoline, for paramagnetic NMR analysis. Related Articles Site-specific tagging proteins with a rigid, small and stable transition metal chelator, 8-hydroxyquinoline, for paramagnetic NMR analysis. J Biomol NMR. 2016 Jan 6; Authors: Yang Y, Huang F, Huber T, Su XC Abstract Design of a paramagnetic metal binding motif in a protein is a valuable way for understanding the function, dynamics and interactions of a protein by paramagnetic...
nmrlearner Journal club 0 01-07-2016 11:10 PM
Site-specific tagging proteins with a rigid, small and stable transition metal chelator, 8-hydroxyquinoline, for paramagnetic NMR analysis
Site-specific tagging proteins with a rigid, small and stable transition metal chelator, 8-hydroxyquinoline, for paramagnetic NMR analysis Abstract Design of a paramagnetic metal binding motif in a protein is a valuable way for understanding the function, dynamics and interactions of a protein by paramagnetic NMR spectroscopy. Several strategies have been proposed to site-specifically tag proteins with paramagnetic lanthanide ions. Here we report a simple approach of engineering a transition metal binding motif via site-specific labelling of a...
nmrlearner Journal club 0 01-06-2016 09:48 AM
[NMR paper] NMR solution structure of a highly stable de novo heterodimeric coiled-coil.
NMR solution structure of a highly stable de novo heterodimeric coiled-coil. Related Articles NMR solution structure of a highly stable de novo heterodimeric coiled-coil. Biopolymers. 2004 Dec 5;75(5):367-75 Authors: Lindhout DA, Litowski JR, Mercier P, Hodges RS, Sykes BD The NMR solution structure of a highly stable coiled-coil IAAL-E3/K3 has been solved. The E3/K3 coiled-coil is a 42-residue de novo designed coiled-coil comprising three heptad repeats per subunit, stabilized by hydrophobic contacts within the core and electrostatic...
nmrlearner Journal club 0 11-24-2010 10:03 PM
[NMR paper] Internal motion time scales of a small, highly stable and disulfide-rich protein: a 1
Internal motion time scales of a small, highly stable and disulfide-rich protein: a 15N, 13C NMR and molecular dynamics study. Related Articles Internal motion time scales of a small, highly stable and disulfide-rich protein: a 15N, 13C NMR and molecular dynamics study. J Biomol NMR. 1999 May;14(1):47-66 Authors: Guenneugues M, Gilquin B, Wolff N, MĂ©nez A, Zinn-Justin S Motions of the backbone C alpha H alpha and threonine C beta H beta bonds of toxin alpha were investigated using natural abundance 13C NMR and molecular dynamics. Measurement...
nmrlearner Journal club 0 08-21-2010 04:03 PM


Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes Rate This Thread
Rate This Thread:

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2022, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 02:32 AM.


Map