Rigid, highly reactive and stable DOTA-like tags containing a thiol-specific phenylsulfonyl pyridine moiety for protein modification and NMR analysis
 

Rigid, highly reactive and stable DOTA-like tags containing a thiol-specific phenylsulfonyl pyridine moiety for protein modification and NMR analysis

Site specific labeling of paramagnetic NMR tags generate valuable structural restraints such as pseudocontact shifts (PCSs) and residual dipolar couplings (RDCs) that can be used to characterize structures, interactions and dynamics of protein-ligand or nucleic acid complexes. Herein, two single-armed DOTA-like tags, BrPSPy-DO3M( S )A-Ln and BrPSPy-6M-DO3M( S )A-Ln, each containing a thiol-specific reacting group, phenylsulfonyl pyridine moiety, are demonstrated as rigid, reactive and stable...

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