[ASAP] Multiple-State Monitoring of SOD1 Amyloid Formation at Single-Residue Resolution by Rheo-NMR Spectroscopy
Multiple-State Monitoring of SOD1 Amyloid Formation at Single-Residue Resolution by Rheo-NMR Spectroscopy
Naoto Iwakawa, Daichi Morimoto, Erik Walinda, Masahiro Shirakawa, and Kenji Sugase
https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.1c02974/20210707/images/medium/ja1c02974_0007.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.1c02974
http://feeds.feedburner.com/~r/acs/jacsat/~4/Q882jnb9IIY
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07-08-2021 11:44 AM
[NMR paper] Multiple-State Monitoring of SOD1 Amyloid Formation at Single-Residue Resolution by Rheo-NMR Spectroscopy
Multiple-State Monitoring of SOD1 Amyloid Formation at Single-Residue Resolution by Rheo-NMR Spectroscopy
Formation of protein aggregates or fibrils entails the conversion of soluble native protein monomers via multiple molecular states. No spectroscopic techniques have succeeded in capturing the transient molecular-scale events of fibrillation in situ. Here we report residue- and state-specific real-time monitoring of the fibrillation of amyotrophic lateral sclerosis-related SOD1 by rheology NMR (Rheo-NMR) spectroscopy. Under moderately denaturing conditions, where NMR signals of folded...
[NMR paper] High-Sensitivity Rheo-NMR Spectroscopy for Protein Studies.
High-Sensitivity Rheo-NMR Spectroscopy for Protein Studies.
High-Sensitivity Rheo-NMR Spectroscopy for Protein Studies.
Anal Chem. 2017 Jun 30;:
Authors: Morimoto D, Walinda E, Iwakawa N, Nishizawa M, Kawata Y, Yamamoto A, Shirakawa M, Scheler U, Sugase K
Abstract
Shear stress can induce structural deformation of proteins, which might result in aggregate formation. Rheo-NMR spectroscopy has the potential to monitor structural changes in proteins under shear stress at the atomic level; however, existing Rheo-NMR methodologies...
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07-01-2017 11:41 AM
[NMR paper] A selective NMR probe to monitor the conformational transition from inactive to active kinase.
A selective NMR probe to monitor the conformational transition from inactive to active kinase.
Related Articles A selective NMR probe to monitor the conformational transition from inactive to active kinase.
ACS Chem Biol. 2014 Sep 23;
Authors: Xie Q, Fulton DB, Andreotti AH
Abstract
Kinases control many aspects of cellular signaling and are therefore therapeutic targets for numerous disease states. Monitoring the conformational changes that drive activation and inactivation of the catalytic kinase core is a challenging...
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09-24-2014 06:22 PM
Site-specific dynamic nuclear polarization of hydration water as a generally applicable approach to monitor protein aggregation
From The DNP-NMR Blog:
Site-specific dynamic nuclear polarization of hydration water as a generally applicable approach to monitor protein aggregation
This article was already published in 2009 but unfortunately I missed it.
Pavlova, A., et al., Site-specific dynamic nuclear polarization of hydration water as a generally applicable approach to monitor protein aggregation. Phys. Chem. Chem. Phys., 2009. 11(31): p. 6833-6839.
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11-21-2013 01:14 AM
Slight mistuning of a cryogenic probe significantly perturbs the water 1H precession frequency
Slight mistuning of a cryogenic probe significantly perturbs the water 1H precession frequency
Abstract A shift of the water proton precession frequency is described that can introduce errors in chemical shifts derived using the water signal as the chemical shift reference. This shift, fs, arises as a consequence of radiation damping when the water proton and detector circuit resonance frequencies differ. Herein it is shown that experimental values of fs, measured as a function of detector circuit tuning offset for 500 and 900 MHz cryogenic probes, are in good agreement with theory. Of...
Rheo-NMR Spectroscopy for Cryogenic-Probe-Equipped NMR Instruments to Monitor Protein Aggregation
Linear Mode
