Real-Time NMR Spectroscopy for Studying Metabolism.
 

Real-Time NMR Spectroscopy for Studying Metabolism.

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Angew Chem Int Ed Engl. 2020 02 03;59(6):2304-2308

Authors: Alshamleh I, Krause N, Richter C, Kurrle N, Serve H, Günther UL, Schwalbe H

Abstract
Current metabolomics approaches utilize cellular metabolite extracts, are destructive, and require high cell numbers. We introduce here an approach that enables the monitoring of cellular metabolism at lower cell numbers by observing the consumption/production of different metabolites over several kinetic data points of up to 48â??hours. Our approach does not influence cellular viability, as we optimized the cellular matrix in comparison to other materials used in a variety of in-cell NMR spectroscopy experiments. We are able to monitor real-time metabolism of primary patient cells, which are extremely sensitive to external stress. Measurements are set up in an interleaved manner with short acquisition times (approximately 7â??minutes per sample), which allows the monitoring of up to 15 patient samples simultaneously. Further, we implemented our approach for performing tracer-based assays. Our approach will be important not only in the metabolomics fields, but also in individualized diagnostics.


PMID: 31730253 [PubMed - indexed for MEDLINE]



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