A detailed knowledge of the complex processes that make cells and organisms alive is fundamental in order to understand diseases and to develop novel drugs and therapeutic treatments. To this aim, biological macromolecules should ideally be characterized at atomic resolution directly within the cellular environment. Among the existing structural techniques, solution NMR stands out as the only one able to investigate at high resolution the structure and dynamic behavior of macromolecules directly...
[ASAP] Polyanions Cause Protein Destabilization Similar to That in Live Cells
Polyanions Cause Protein Destabilization Similar to That in Live Cells
https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.0c00889/20210226/images/medium/bi0c00889_0009.gif
Biochemistry
DOI: 10.1021/acs.biochem.0c00889
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02-28-2021 06:53 AM
A Fluorescent Probe with Improved Water SolubilityPermits the Analysis of Protein S-DepalmitoylationActivity in Live Cells
A Fluorescent Probe with Improved Water SolubilityPermits the Analysis of Protein S-DepalmitoylationActivity in Live Cells
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b00835/20171018/images/medium/bi-2017-00835x_0005.gif
Biochemistry
DOI: 10.1021/acs.biochem.7b00835
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10-19-2017 08:03 AM
[NMR paper] The first successful observation of in-cell NMR signals of DNA and RNA in living human cells.
The first successful observation of in-cell NMR signals of DNA and RNA in living human cells.
Related Articles The first successful observation of in-cell NMR signals of DNA and RNA in living human cells.
Phys Chem Chem Phys. 2017 Oct 12;:
Authors: Yamaoki Y, Kiyoishi A, Miyake M, Kano F, Murata M, Nagata T, Katahira M
Abstract
In order to understand intracellular biological events, information on the structure, dynamics and interaction of proteins and nucleic acids in living cells is of crucial importance. In-cell NMR is a...
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10-14-2017 02:04 AM
Visualizing RNA-protein Interactions in Live Human Cells - Bioscience Technology
Visualizing RNA-protein Interactions in Live Human Cells - Bioscience Technology
http://www.bionmr.com//t3.gstatic.com/images?q=tbn:ANd9GcRoPawKAwV63VcpQ8t5e9ZjpOvT0S-FLAVv5fJ1CFHJeDztic5RkQBERPvhe9MhZmtvPn17oDdy
Bioscience Technology
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Visualizing RNA-protein Interactions in Live Human Cells
Bioscience Technology
UAlbany chemists Alan Chen Ph.D, Maksim Royzen Ph.D, and Alex Shekhtman Ph.D have combined their labs on a collaborative project aimed at visualizing RNA-protein interactions inside live human cells by using multi-dimensional NMR (nuclear ...
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06-03-2017 11:49 AM
Progression of NMR studies of membrane-active peptides from lipid bilayers to live cells
Progression of NMR studies of membrane-active peptides from lipid bilayers to live cells
Publication date: Available online 27 December 2014
Source:Journal of Magnetic Resonance</br>
Author(s): M.-A. Sani , F. Separovic</br>
Understanding the structure of membrane-active peptides faces many challenges associated with the development of appropriate model membrane systems as the peptide structure depends strongly on the lipid environment. This perspective provides a brief overview of the approach taken to study antimicrobial and amyloid peptides in phospholipid...
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12-28-2014 10:29 PM
[NMR paper] Live Cell NMR.
Live Cell NMR.
Related Articles Live Cell NMR.
Annu Rev Biophys. 2014 May 6;43:171-192
Authors: Freedberg DI, Selenko P
Abstract
Ever since scientists realized that cells are the basic building blocks of all life, they have been developing tools to look inside them to reveal the architectures and mechanisms that define their biological functions. Whereas "looking into cells" is typically said in reference to optical microscopy, high-resolution in-cell and on-cell nuclear magnetic resonance (NMR) spectroscopy is a powerful method...
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06-05-2014 05:51 PM
[NMR paper] Atomic-resolution monitoring of protein maturation in live human cells by NMR.
Atomic-resolution monitoring of protein maturation in live human cells by NMR.
Atomic-resolution monitoring of protein maturation in live human cells by NMR.
Nat Chem Biol. 2013 Mar 3;
Authors: Banci L, Barbieri L, Bertini I, Luchinat E, Secci E, Zhao Y, Aricescu AR
Abstract
We use NMR directly in live human cells to describe the complete post-translational maturation process of human superoxide dismutase 1 (SOD1). We follow, at atomic resolution, zinc binding, homodimer formation and copper uptake, and discover that copper chaperone for...
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03-05-2013 03:25 PM
[NMR paper] Quantitative comparison of protein dynamics in live cells and in vitro by in-cell (19)F-NMR.
Quantitative comparison of protein dynamics in live cells and in vitro by in-cell (19)F-NMR.
Quantitative comparison of protein dynamics in live cells and in vitro by in-cell (19)F-NMR.
Chem Commun (Camb). 2013 Feb 26;
Authors: Takaoka Y, Kioi Y, Morito A, Otani J, Arita K, Ashihara E, Ariyoshi M, Tochio H, Shirakawa M, Hamachi I
Abstract
Here we describe how a (19)F-probe incorporated into an endogenous protein by a chemical biology method revealed protein dynamics. By explicit determination of ligand-bound and unbound structures with...
Radio Signals from Live Cells: The Coming of Age of In-Cell Solution NMR
Linear Mode
