Quantitative Measurement of Multiprotein Nanoparticle Interactions Using NMR Spectroscopy
 

Quantitative Measurement of Multiprotein Nanoparticle Interactions Using NMR Spectroscopy

An effective intensity-based reference is a cornerstone for quantitative nuclear magnetic resonance (NMR) studies, as the molecular concentration is encoded in its signal. In theory, NMR is well suited for the measurement of competitive protein adsorption onto nanoparticle (NP) surfaces, but current referencing systems are not optimized for multidimensional experiments. Presented herein is a simple and novel referencing system using ^(15)N tryptophan (Trp) as an external reference for ¹H-^(15)N...

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