Purification of cloned trypanosomal calmodulin and preliminary NMR studies.
 

Purification of cloned trypanosomal calmodulin and preliminary NMR studies.

Related Articles Purification of cloned trypanosomal calmodulin and preliminary NMR studies.

J Chromatogr. 1991 Feb 22;539(2):501-5

Authors: Sweeney PJ, Walker JM, Reid DG, Elshourbagy N

Cloned trypanosomal calmodulin was expressed in Escherichia coli and purified to homogeneity using hydrophobic interaction chromatography on phenyl-Sepharose. The purified protein was subjected to NMR analysis which allows detailed changes to be observed when, firstly, calcium, and secondly, the drug calmidazolium bind. These spectral changes are the result of conformational changes in the protein and proximity effects due to the drug.

PMID: 2045459 [PubMed - indexed for MEDLINE]



Source: PubMed